AI Article Synopsis
- Yield and productivity are essential for the economic success of bioprocesses, particularly in metabolic engineering, where the goal is to enhance target metabolite production through genetic modifications.
- Genetic manipulations can often lead to decreased productivity due to issues with growth, but dynamic control of metabolic fluxes can improve product yield in anaerobic fermentations of Escherichia coli.
- An integrated computational model has been developed to dynamically control gene expression using a genetic toggle switch, which, when applied to E. coli metabolism, significantly boosts bioprocess productivity.
Article Abstract
Yield and productivity are critical for the economics and viability of a bioprocess. In metabolic engineering, the main objective is the increase of a target metabolite production through genetic engineering. However, genetic manipulations usually result in lower productivity due to growth impairment. Previously, it has been shown that the dynamic control of metabolic fluxes can increase the amount of product formed in an anaerobic batch fermentation of Escherichia coli. In order to apply this control strategy, the genetic toggle switch is used to manipulate key fluxes of the metabolic network. We have designed and analyzed an integrated computational model for the dynamic control of gene expression. This controller, when coupled to the metabolism of E. coli, resulted in increased bioprocess productivity.
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| http://dx.doi.org/10.1016/j.ymben.2008.06.004 | DOI Listing |
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