Objective: To explore the appropriate amount of template DNA for Sinofiler Kit.
Methods: The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
Results: It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
Conclusion: Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
template dna
16
appropriate amount
12
amount template
12
quantitative pcr
12
dna sinofiler
8
sinofiler kit
8
real time
8
time quantitative
8
dna
5
[analysis appropriate
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!