AI Article Synopsis
- * Using specific differentiation markers (CD19, CD20, CD34), we sorted cells from patient samples and found they could successfully re-engraft and proliferate in mice through multiple generations.
- * All maturation stages of the blasts were capable of restoring the full leukemic phenotype in vivo, suggesting that these cells behave similarly to normal B precursor cells and informing our understanding of leukemia-propagating stem cells in childhood ALL.
Article Abstract
We examined the leukemic stem cell potential of blasts at different stages of maturation in childhood acute lymphoblastic leukemia (ALL). Human leukemic bone marrow was transplanted intrafemorally into NOD/scid mice. Cells sorted using the B precursor differentiation markers CD19, CD20, and CD34 were isolated from patient samples and engrafted mice before serial transplantation into primary or subsequent (up to quaternary) recipients. Surprisingly, blasts representative of all of the different maturational stages were able to reconstitute and reestablish the complete leukemic phenotype in vivo. Sorted blast populations mirrored normal B precursor cells with transcription of a number of stage-appropriate genes. These observations inform a model for leukemia-propagating stem cells in childhood ALL.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572185 | PMC |
| http://dx.doi.org/10.1016/j.ccr.2008.05.015 | DOI Listing |
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