Cytomegalovirus infection (CMVI) is becoming the serious medical-social problem both in clinical-epidemiological aspects and in connection with demographic consequences. One of the necessary conditions for solving this problem is the organization of regular and comprehensive epidemiological surveillance for CMVI. Thereby, improvement of methods of diagnostics is becoming the most actual issue. Immunoenzyme assay-based test-systems "ECOlab-CMV-IgM" and "ECOlab-CMV-IgG" which are able to detect IgM and IgG for CMVI diagnostics and assessment of IgG titers dynamics were presented. Their high specificity and sensitivity were shown.
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Biosensors (Basel)
November 2018
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
Grapevine leafroll-associated virus 3 (GLRaV-3) is one of the main pathogens of grapes, causing a significant loss in yield and decrease in quality for this agricultural plant. For efficient widespread control of this infection, rapid and simple analytical techniques of on-site testing are requested as a complementary addition for the currently applied hybridization (PCR) and immunoenzyme (ELISA) approaches. The given paper presents development and approbation of the immunochromatographic assay (ICA) for rapid detection of GLRaV-3.
View Article and Find Full Text PDFMycotoxin Res
February 2019
Chair of Food Safety, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich (LMU), Schoenleutnerstr. 8, 85764, Oberschleissheim, Germany.
The applicability of enzyme immunoassays (EIA) for aflatoxin M (AFM), ochratoxin A (OTA) and zearalenone (ZEN) to analyse these toxins in donkey milk (Equus asinus) was studied. For AFM and OTA analysis, milk could be analysed by EIA without sample pretreatment. For ZEN, heat treatment at 78 °C for 30 min prior EIA analysis was required to avoid false positives.
View Article and Find Full Text PDFIntroduction: The paper presents the results of studying the role of interleukins 4 and 6 in the pathogenesis of periodontal tissue diseases, specifically, in periodontitis, gingivitis and alveolitis.
The Aim: To study the nature of participation of IL-4 and IL-6 in the mechanisms of development of periodontitis, gingivitis and alveolitis.
Materials And Methods: Studies were carried out on 80 nonlinear male rats with a body weight of 200.
Zh Mikrobiol Epidemiol Immunobiol
May 2016
Aim: Comparative study of antigenic properties of recombinant proteins OsPCgar and OsPCafz and recombinant chimeric polypeptide OspCgar+afrz, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia.
Materials And Methods: Recombinant chimeric polypeptide OSpCgar+af, and recombinant mature proteins OSPCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells; purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients.
Klin Lab Diagn
January 2016
EKOlab, 142424, Elektrogorsk, Russia.
The immune chip is developed for detecting immunoglobulins G to agents of four infections of TORCH-complex (toxoplasmosis, German measles, cytomegalovirus and herpes viral infections) on the basis of FOSFANtm technology. The sensitivity and specificity of simultaneous detection of IgG on immune chip were comparable with indices of commercial immunoenzyme test-systems, including under analysis of standard panels of serums. This permits considering derived results as a basis for development of commercial multiplex test intended for highly productive screening of TORCH-infections in clinical laboratory.
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