Introduction: A Polycomb group repressor protein named BMI1 represses the genes that induce cellular senescence and cell death, and it can contribute to cancer when improperly expressed. We aimed to evaluate expression of BMI1 gene in bladder tumors.
Materials And Methods: Tissue specimens containing bladder tumor were evaluated and compared with intact tissues from tumor margins and normal bladders. There were 40 tumor specimens of patients with transitional cell carcinoma of the bladder, 20 tumor-free tissues taken from the margin of the tumors, and 8 specimens from patients without tumor. Specific primers for BMI1 and B2M (as an internal control) were used for reverse transcript polymerase chain reaction technique. The production and distribution of BMI1 protein was also examined by western blotting and immunohistochemistry techniques.
Results: Polymerase chain reaction generated a 683-bp product, corresponding to the expected size of BMI1 amplified region. The identity of the amplified fragment was then confirmed by direct DNA sequencing. The mean of expression of BMI1 detected in tumor tissues was significantly higher than that in intact tissues, and there was also a significant association between the mean of gene expression and the stage of malignancy (P < .001). The expression of BMI1 at protein level was further confirmed by western blotting and immunohistochemistry.
Conclusion: BMI1 is a potent repressor of retinoblastoma and p53 pathways, and hence, elucidating its role in tumorigenesis is very important. We reported for the first time the expression of BMI1 and its correlation with incidence and progress of bladder tumors.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Targeting the BMI1-Noxa axis by Dioscin induces apoptosis in oral squamous cell carcinoma cells.
J Cancer
January 2025
School of Stomatology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
Article Synopsis
- Dioscin is a natural steroidal saponin that shows antitumor effects by triggering apoptosis in various cancers, particularly through the Noxa protein.
- The study found that Dioscin enhances Noxa expression by reducing BMI1 levels, leading to increased tumor cell death in oral squamous cell carcinoma (OSCC).
- Additionally, Dioscin demonstrates effective tumor suppression in animal models, offering potential new strategies for treating OSCC.
The polycomb protein complex interacts with GATA-6/PPARα to inhibit α-MHC expression.
Dev Growth Differ
December 2024
Department of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
Transcription factors collaborate with epigenetic regulatory factors to orchestrate cardiac differentiation for heart development, but the underlying mechanism is not fully understood. Here, we report that GATA-6 induces cardiac differentiation but peroxisome proliferator-activated receptor α (PPARα) reverses GATA-6-induced cardiac differentiation, possibly because GATA-6/PPARα recruits the polycomb protein complex containing EZH2/Ring1b/BMI1 to the promoter of the cardiac-specific α-myosin heavy chain (α-MHC) gene and suppresses α-MHC expression, which ultimately inhibits cardiac differentiation. Furthermore, Ring1b ubiquitylates PPARα and GATA-6.
View Article and Find Full Text PDFBerberine Derivative B68 Promotes Tumor Immune Clearance by Dual-Targeting BMI1 for Senescence Induction and CSN5 for PD-L1 Degradation.
Adv Sci (Weinh)
December 2024
Shanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Promoting tumor cell senescence arrests the cell cycle of tumor cells and activates the immune system to eliminate these senescent cells, thereby suppressing tumor growth. Nevertheless, PD-L1 positive senescent tumor cells resist immune clearance and possess the ability to secret various cytokines and inflammatory factors that stimulate the growth of tumor cells. Consequently, drugs capable of both triggering senescence in tumor cells and concurrently diminishing the expression of PD-L1 to counteract immune evasion are urgently needed.
View Article and Find Full Text PDFNeuron stress-related genes serve as new biomarkers in hypothalamic tissue following high fat diet.
Front Endocrinol (Lausanne)
December 2024
Medical Research Center, Affiliated Hospital 2 of Nantong University, Nantong, China.
Objective: Energy homeostasis is modulated by the hypothalamic is essential for obesity progression, however, the gene expression profiling remains to be fully understood.
Methods: GEO datasets were downloaded from the GEO website and analyzed by the R packages to obtain the DEGs. And, the WGCNA analysis and PPI networks of co-expressed DEGs were designed using STRING to get key genes.
Background: B-cell-specific Moloney murine leukemia virus integration site 1 (BMI1) and Fas ligand (FasL) are two critical stemness genes believed to play a role in the development of colorectal cancer (CRC). This study aimed to investigate the expression levels of these genes in primary CRC tumors to assess their correlation with cancer progression and prognosis.
Methods: The relative expression levels of BMI1 and FasL were analyzed using real-time polymerase chain reaction in 100 primary CRC tumor samples along with paired adjacent non-cancerous tissues.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!