The model of a continuous-stirred tank biochemical reactor was developed in which the instant uptake rate of substrate was used. The solutions of the model found for the oxidation of phenol by Pseudomonas putida fitted the experimental data better than the results obtained from the models cited in the literature. The model enables control of the culture parameters so that the unwanted washout of the biomass from the bioreactor can be avoided. A review of the models cited in the literature is also presented.
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http://dx.doi.org/10.1002/bit.260310303 | DOI Listing |
J Biol Eng
January 2025
Biochemical Engineering (AVT.BioVT), RWTH Aachen University, Aachen, Germany.
Background: For process development in mammalian cell cultivations, scale-up approaches are essential. A lot of studies concern the scale transfer between different-sized stirred tank reactors. However, process development usually starts in even smaller cultivation vessels like microtiter plates or shake flasks.
View Article and Find Full Text PDFInt J Pharm
January 2025
School of Mechanical and Mechatronic Engineering, University of Technology Sydney (UTS), 15 Broadway, Ultimo, New South Wales 2007, Australia. Electronic address:
Nanocarrier-based dry powders for lung disease treatment are crucial, with in vitro and in silico research being pivotal to their success. This study introduces a method for creating Tiotropium-bromide liposomal inhalation dry powder, termed "Trojan-particles," utilizing thin-film hydration and spray-drying with lactose-arginine carriers. Encapsulating tiotropium-bromide in nanoliposomes enhances lung treatment via liposomes' unique features.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
Laboratory of Atmospheric Environment and Pollution Control (LAEPC), Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
Heavy air pollution is now a serious public health issue. Many studies have shown strong connections between ozone (O) with the occurrence and development of various respiratory diseases. However, the exact mechanism is still a matter of debate.
View Article and Find Full Text PDFPrep Biochem Biotechnol
January 2025
Environmental Technology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, India.
The study employed batch shake flasks to evaluate the impact of various nitrogen sources, phosphate levels, and sodium acetate (Na-acetate) on the growth and metabolite production. Adding Na-acetate to the medium resulted in significant improvements in critical metabolites. In shake flask experiments, this led to a cell dry weight (CDW) of 1.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
KU Leuven, Department of Chemical Engineering, Chemical and Biochemical Reactor Engineering and Safety (CREaS), Celestijnenlaan 200F, 3001 Leuven, Belgium. Electronic address:
The fabrication of objects with complex shape and geometry has been greatly facilitated with the advancements in additive manufacturing. While synthetic polymers like ABS and PLA have found widespread use in extrusion 3D printing, other biobased thermoplastics that are both biodegradable and biocompatible could offer strategic advantages over traditional synthetic materials. In this work dextran of low (20 kDa) and medium (40 kDa) molecular weight (MW) was modified with palmitic acid to obtain meltable polymers for extrusion 3D printing/fused deposition modeling additive manufacturing.
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