Digital holography of total internal reflection.

Opt Express

Digital Holography and Microscopy Laboratory, Dept. of Physics, University of South Florida, Tampa, FL 33620, USA.

Published: June 2008

AI Article Synopsis

  • A new microscopy technique called total internal reflection holographic microscopy (TIRHM) is introduced for better imaging.
  • It uses quantitative phase microscopy through digital holography to analyze the phase profile of light during total internal reflection, influenced by nearby materials.
  • The study includes theoretical modeling of imaging characteristics and experimental demonstration of its imaging capabilities.

Article Abstract

We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.

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http://dx.doi.org/10.1364/oe.16.009811DOI Listing

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