Three-wavelength digital holography is applied to obtain surface height measurements over several microns of range, while simultaneously maintaining the low noise precision of the single wavelength phase measurement. The precision is preserved by the use of intermediate synthetic wavelength steps generated from the three wavelengths and the use of hierarchical optical phase unwrapping. As the complex wave-front of each wavelength can be captured simultaneously in one digital image, real-time performance is achievable.
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http://dx.doi.org/10.1364/oe.16.009753 | DOI Listing |
We propose a multi-wavelength digital holography based on Kramers-Kronig (KK) relations, introducing a unified angle-multiplexing multi-wavelength KK model to overcome the accuracy and resolution limitations of angle-multiplexing techniques. By linking the real and imaginary parts of the multi-wavelength complex function via the KK relation, the method captures object light waves with the full effective bandwidth from a single interferogram and reference wave intensity. This method greatly improves spectral utilization and measurement accuracy in multi-wavelength interference.
View Article and Find Full Text PDFLasers Med Sci
March 2024
Department of Dermatology, University of California, Irvine, 118 Medical Surge I, Irvine, CA, 92697, USA.
Objective of the study is to assess the effects of wound healing with a commercially available light emitting diode (LED) photo biomodulation (PBM) device that emits three wavelengths (465, 640 and 880nm) after ablative fractional laser (AFL) treatment to healthy skin on the bilateral inner biceps. We conducted a prospective intraindividual randomized controlled study with 25 volunteers. AFL treatment was performed on healthy skin of the bilateral inner biceps.
View Article and Find Full Text PDFA three-wavelength photoelasticity method is proposed to simplify the optical setup and speed up data acquisition. By recording six intensity images with circularly polarized illuminations of three close wavelengths, the phase retardation and corresponding inner stress can be computed accurately with a correspondingly developed computational algorithm. Since the mechanical rotations of wave plates and polarizers required by classic photoelasticity techniques are avoided, the data acquisition of this proposed method is very speedy, and measurement of a dynamic sample can be achieved with a very simple and compact optical setup.
View Article and Find Full Text PDFBiomed Opt Express
November 2020
Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv 69978, Israel.
We report a cross-talk free simultaneous three-wavelength digital holographic microscopy setup for spectroscopic imaging of biological cells during flow. The feasibility of the proposed measurement technique is demonstrated on erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) molecule absorption on wavelength. From the spectroscopic quantitative phase profiles of cells acquired during flow in a microfluidic device, we decoupled the refractive index and the physical thickness.
View Article and Find Full Text PDFWe report on the new technical realization of single-shot color digital holographic microscopy. For this application, we propose to use an original three-wavelength red, green, and blue laser based on the Nd:YAG active element with sequential parametric downconversion and upconversion of optical frequencies into red (634 nm), green (532 nm), and blue (451 nm) spectral intervals. This light source provides high-power short (∼10 ns) pulses and enables simultaneous formation of three color interference patterns in a two-path interferometer.
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