AI Article Synopsis
- Five dermatophyte species were tested for their response to Penicillium chrysogenum antifungal protein (PAF) and fluconazole (FCZ), with varying minimum inhibitory concentrations (MICs) for each.
- PAF exhibited a fungicidal effect at concentrations of 200 microg ml(-1) and inhibited growth at levels between 50 and 200 microg ml(-1), while FCZ showed MICs ranging from 0.25 to 128 microg ml(-1).
- The combination of PAF and FCZ demonstrated synergistic or additive effects against most fungi tested, except for M. gypseum, which did not show any interactions.
Article Abstract
Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 microg ml(-1) when it was used alone, or at constant concentration (100 microg ml(-1)) in combination with FCZ at from 0.25 to 32 microg ml(-1). The MICs for FCZ were found to be between 0.25 and 128 microg ml(-1). PAF caused a fungicidal effect at 200 microg ml(-1) and reduced growth at between 50 and 200 microg ml(-1). Total growth inhibition with fungistatic activity was detected at 64 microg ml(-1) of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 microg ml(-1) FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10482-008-9263-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ovarian steroid hormone secretion by human granulosa cells after supplementation of sambucus nigra l. extract.
Physiol Res
November 2021
AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic. Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
Beneficial effects of Sambucus nigra L. (black elder) as a traditional medicine have been associated with the phytoconstituents including polyphenols, terpenes and lectins. Various antioxidant rich natural products have also been implicated with improvement of reproductive health and fertility, however, the effect of Sambucus nigra on the ovarian cell functions has not been investigated yet.
View Article and Find Full Text PDFAntioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa cells HGL5 in vitro.
Physiol Res
November 2021
Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17beta-estradiol and progesterone, and human transforming growth factor-beta2 (TGF-beta2) and TGF-beta2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 microg.ml-1.
View Article and Find Full Text PDFIdentification of Bioactive Compounds from Marine Natural Products and Exploration of Structure-Activity Relationships (SAR).
Antibiotics (Basel)
March 2021
School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
Marine natural products (MNPs) have been an important and rich source for antimicrobial drug discovery and an effective alternative to control drug resistant infections. Herein, we report bioassay guided fractionation of marine extracts from sponges , and that led us to identify novel compounds with antimicrobial properties. Tertiary amines or quaternary amine salts: aniline , benzylamine , tertiary amine and , and quaternary amine salt , along with three known compounds () were isolated from a crude extract and MeOH eluent marine extracts.
View Article and Find Full Text PDFDiclofenac-induced cytotoxicity in cultured carp leukocytes.
Physiol Res
December 2020
Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno,Czech Republic.
Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment.
View Article and Find Full Text PDFOvarian steroid hormone secretion activity examined after supplementation of green tea extract.
Physiol Res
December 2017
Department of Life Science and Bioinformatics, Assam University, Silchar, India, Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
This study aimed at examining the secretion activity of steroid hormones progesterone and 17beta-estradiol by porcine ovarian granulosa cells after addition of green tea extract. Granulosa cells were incubated with green tea extract (at doses of 0.01, 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!