Recent studies show that ethyl glucuronide (EtG) can be decomposed by bacteria; whilst so far no degradation of ethyl sulphate (EtS) has been observed. In the present study, in vitro experiments with bacterial colonies were performed. Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isolated from autopsy material (liver, heart blood, urine, ascites, pericardial fluid, pleural fluid) tested for beta-glucuronidase activity, and three bacterial strains were added to nutrient-deficient medium containing EtG and/or EtS and incubated at 36 +/- 1 degrees C. Samples were taken after various intervals up to 11 days, and EtG and EtS were determined by electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS). EtG was degraded by E. coli and C. sordellii--complete degradation occurred in the range of 3-4 days--and these bacteria exhibited beta-glucuronidase activity. EtS was not affected within 11 days of incubation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00414-008-0229-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The influence of different crosslinking agents onto the physical properties, integration behavior and immune response of collagen-based barrier membranes.
Front Bioeng Biotechnol
January 2025
Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany.
This study investigates the mechanical properties as well as and cyto- and biocompatibility of collagen membranes cross-linked with glutaraldehyde (GA), proanthocyanidins (PC), hexamethylendiisocyanate (HMDI) and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EC/NHS). A non-crosslinked membrane was used as reference control (RF). The initial cytotoxic analyses revealed that the PC, EC, and HMDI crosslinked membranes were cytocompatible, while the GA crosslinked membrane was cytotoxic and thus selected as positive control in the further study.
View Article and Find Full Text PDFUrinary phthalate metabolites associated with increased prevalence of gallstone disease in U.S. adults: data from the NHANES study.
BMC Public Health
January 2025
The First Hospital of Jilin University, No.1 Xinmin Street, Changchun, 130012, China.
Background: Phthalate exposure has been hypothesized to influence cholesterol metabolism and gallstone pathogenesis, but previous studies are limited. We aimed to examine the associations between urinary phthalate metabolites and prevalence of gallstone disease in a nationally representative sample.
Methods: We analyzed data on 1,696 adults aged ≥ 30 years from the National Health and Nutrition Examination Survey (NHANES) 2017-2018.
Effect of HO induced oxidative stress on volatile organic compounds in differentiated 3T3-L1 cells.
Sci Rep
January 2025
Department of Nutritional Sciences, Auburn University, Auburn, AL, 36849, USA.
Oxidative stress (OS) refers to the disruption in the balance between free radical generation and antioxidant defenses, leading to potential tissue damage. Reactive oxygen species (ROS) can interact with biological components, triggering processes like protein oxidation, lipid peroxidation, or DNA damage, resulting in the generation of several volatile organic compounds (VOCs). Recently, VOCs provided new insight into cellular metabolism and can serve as potential biomarkers.
View Article and Find Full Text PDFThermally and Base-Triggered "Debond-on-Demand" Chain-Extended Polyurethane Adhesives.
Macromolecules
January 2025
Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6AD, U.K.
A series of novel chain-extended polyurethanes (CEPUs) featuring degradable sulfonyl ethyl urethane chain-extenders that permit degradation under base-triggered conditions to afford "debond-on-demand" elastomeric adhesives are reported. Exposure of the CEPUs to -butylammonium fluoride (TBAF) triggered the degradation of the sulfonyl ethyl urethane chain-extenders. Lap shear adhesion tests of the CEPUs exposed to TBAF revealed reductions in shear strength of up to 65% for both aluminum and glass substrates, from 2.
View Article and Find Full Text PDFHigh-Throughput Screening Strategy and Metal-Organic Framework-Based Multifunctional Controlled-Release Nanomaterial for Osteoarthritis Therapy.
ACS Nano
January 2025
Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
Osteoarthritis (OA) is a prevalent degenerative disease that lacks effective therapy. Oxidative stress is one of the major factors contributing to OA; however, treatments targeting oxidative stress are still lacking. In the current study, we established an oxidative stress-induced cell death model in chondrocytes and screened drugs that may suppress oxidative stress-induced cell death.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!