Invasion of bacteria into nonphagocytic host cells is an important pathogenicity factor for escaping the host defence system. Gram-positive organisms, for example Staphylococcus aureus and Listeria monocytogenes, are invasive in nonphagocytic cells, and this mechanism is discussed as an important part of the infection process. Uropathogenic Escherichia coli and Staphylococcus saprophyticus can cause acute and recurrent urinary tract infections as well as bloodstream infections. Staphylococcus saprophyticus shows strong adhesion to human urinary bladder carcinoma and Hep2 cells and expresses the 'Microbial Surface Components Recognizing Adhesive Matrix molecule' (MSCRAMM)-protein SdrI with collagen-binding activity. MSCRAMMs are responsible for adhesion and collagen binding in S. aureus and are discussed as an important pathogenicity factor for invasion. To investigate internalization in S. aureus, several fluorescence activated cell sorting (FACS) assays have been described recently. We used a previously described FACS assay, with slight modifications, in addition to an antibiotic protection assay and transmission electron microscopy to show that S. saprophyticus ATCC 15305 and the wild-type strain 7108 were internalized into the human urinary bladder carcinoma cell line 5637. The discovery of the internalization of S. saprophyticus may be an important step for understanding the pathogenicity of recurrent infections caused by this organism.
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http://dx.doi.org/10.1111/j.1574-6968.2008.01218.x | DOI Listing |
Front Microbiol
November 2024
Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.
Introduction: One of the most prevalent and recurrent infectious diseases that can range from moderate to fatal is urinary tract infection (UTI). Broad-spectrum antibiotics are the only management strategy for UTIs in ambulators and hospital stays. Due to the ongoing emergence of antibiotic resistance among uropathogens, there is a need for proper selection of antibiotics for empirical therapy against UTIs.
View Article and Find Full Text PDFFront Vet Sci
November 2024
Institute of Innovative Traditional Chinese Medications, Hunan Academy of Chinese Medicine, Hunan, China.
Res Vet Sci
January 2025
Dipartimento di Medicina Veterinaria e Scienze Animali - DIVAS, Università degli Studi di Milano, 26900 Lodi, Italy; Laboratorio di Malattie Infettive degli Animali (MiLab), Università degli Studi di Milano, 26900 Lodi, Italy. Electronic address:
Staphylococcus aureus is the main etiologic agent of contagious dairy cow mastitis, while non-aureus staphylococci and mammaliicocci (NASM) are the bacteria most frequently isolated from milk. Beyond their impact on animal health, NASM can harbor antimicrobial resistance (AMR) genes with potential for bidirectional transfer with S. aureus, and methicillin-resistant (MR) staphylococci (MRS) can raise significant One Health concerns.
View Article and Find Full Text PDFVet Sci
November 2024
Tierklinik Neu-Isenburg, Carl-Friedrich-Gauß-Straße 5, 63263 Neu-Isenburg, Germany.
Many bacteria, including methicillin-resistant staphylococci and opportunistic pathogens such as (.) species, in particular members of the - () complex, are known to survive in environmental settings. This increases the risk of bacterial spreading and transmission to animals and humans, especially in institutions with a high animal population density.
View Article and Find Full Text PDFHeliyon
November 2024
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Desert plants possess untapped potential for medicinal applications due to their rich phytochemical profiles. However, they need to be more explored. Thus, this study integrates advanced analytical, biochemical, and molecular techniques to investigate the phytochemical composition and biological activities (antimicrobial and antioxidant) of four desert plants (, and , collected from Wadi Sannor, Beni-Suef Governorate, Egypt, in March 2021.
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