A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE x NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE x NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B x NHS), T4-BSA- B x NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.
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