AI Article Synopsis
- - Endothelial cells are suggested to play a significant role in the formation of scars, although the exact reasons for scar development are not fully understood.
- - Researchers developed a new technique to isolate endothelial cells from hypertrophic scars by separating them from the surrounding tissue and other cell types.
- - The isolated cells were confirmed to be endothelial in origin through their unique physical features and the expression of specific mRNA and proteins, making this method valuable for further research on endothelial cells in scar formation.*
Article Abstract
Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation.
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| http://dx.doi.org/10.1080/10623320802125169 | DOI Listing |
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