Autoregulation of swrAA and motility in Bacillus subtilis.

J Bacteriol

Dipartimento di Genetica e Microbiologia A Buzzati-Traverso, Università degli Studi di Pavia, Via Ferrata 1, 27100 Pavia, Italy.

Published: August 2008

AI Article Synopsis

  • The gene swrAA in Bacillus subtilis is essential for swarming migration and is regulated by two promoters, one depending on the sigD gene and another on sigA, which becomes active under specific conditions.
  • Expression of swrAA is part of a positive feedback loop, where its transcription is enhanced by SwrAA itself, promoting further expression and motility.
  • Full motility in B. subtilis requires both the response regulator DegU and SwrAA, as the lack of the degS-degU two-component system prevents SwrAA's positive effects on swimming and swarming.

Article Abstract

We demonstrate that transcription of the gene swrAA, required for swarming migration in Bacillus subtilis, is driven by two promoters: a sigD-dependent promoter and a putative sigA-dependent promoter, which is inactive during growth in liquid Luria-Bertani medium and becomes active in the presence of the phosphorylated form of the response regulator DegU or on semisolid surfaces. Since sigD transcription is enhanced by SwrAA, this finding reveals that swrA expression is controlled by a positive feedback loop. We also demonstrate that the positive action of SwrAA in swimming and swarming motility is prevented in strains carrying a deletion of the two-component system degS-degU and that this effect is independent of swrAA transcription. Therefore, both DegU and SwrAA must be present to achieve full motility in B. subtilis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519373PMC
http://dx.doi.org/10.1128/JB.00455-08DOI Listing

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