Background: Flow cytometric analysis of peripheral blood dendritic cells (PBDCs) and their myeloid (mDCs) and plasmacytoid (pDCs) subsets is a less invasive procedure that is acquiring growing clinical relevance. Because dendritic cells (DCs) lack unique lineage markers, current methods that are based on 3- or 4-color assays do not allow multiparametric analysis of DC subsets. In this study a dedicated 6-color assay was developed.
Methods: mDCs and pDCs were counted and characterized for the expression of activation/maturation markers by using a single-platform 6-color assay. Whole-blood samples from 20 healthy controls were directly stained with either CD80-FITC/CD40-PE/lineage-PerCP-Cy5.5/CD123-PE-Cy7/CD11c-APC/HLA-DR-APC-Cy7 or CD86-FITC/CD83-PE/lineage-PerCP-Cy5.5/CD123-PE-Cy7/CD11c-APC/HLA-DR-APC-Cy7 combination, in the presence of commercial fluorospheres. A dual-platform 3-color assay currently in use was run in parallel for comparison.
Results: The 6-color assay provided mDCs and pDCs counts similar to counts obtained by the 3-color assay. Only the 6-color assay could show differential expression of activation markers by mDCs and pDCs, with pDCs expressing lower levels of costimulatory molecules and HLA-DR, but higher levels of CD83.
Conclusions: The 6-color assay described here may be a sensitive tool for assessing possible variations in the number and features of mDCs and pDCs whose reciprocal balance is critical in understanding the more detailed orchestration of immune responses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cyto.b.20434 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of advanced oxidation process on biological treatment of spent fermentation broth.
Environ Sci Pollut Res Int
January 2025
Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, 248005, Dehradun, Uttarakhand, India.
The present study aimed to establish the feasibility of the wastewater treatment process generated from an oleaginous fermentation plant. Treatment of spent fermentation broth (SFB) poses significant environmental challenges due to its high organic load, recalcitrant compounds, and potential toxicity. The synergistic effects of combining ozone-based advanced oxidation process (O-AOP) with biological treatment for the efficient degradation of pollutants in spent fermentation broth.
View Article and Find Full Text PDFCharacterization of a Mycoplasma hyopneumoniae aerosol infection model in pigs.
Vet Microbiol
December 2024
Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA; Swine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA. Electronic address:
Article Synopsis
- - The study aimed to create a model to understand how Mycoplasma hyopneumoniae causes respiratory disease in pigs, focusing on its effects on infection, immune response, and disease progression.
- - Four groups of pigs were exposed to different aerosol doses of the bacteria, with secretions collected and analyzed at various time points to assess infection and immune response.
- - Findings indicated that all pigs became infected regardless of the dose, but only high-dose exposures led to severe clinical symptoms and lung damage, demonstrating the model's potential for testing treatments and prevention strategies.
Flow cytometry analysis of myeloid derived suppressor cells using 6 color labeling.
Methods Cell Biol
November 2024
Clinical Biochemistry Service, Hospital Universitario Virgen Macarena, University of Seville, Seville, Spain; Department of Medical Biochemistry and Molecular Biology and Immunology, Medical School, Virgen Macarena University Hospital, University of Seville, Seville, Spain; Institute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, Seville, Spain. Electronic address:
Myeloid-derived suppressor cells (MDSCs) encompass a diverse population of immature myeloid cells categorized into granulocytic and monocytic groups. These cells exert immune-suppressive functions within the tumor microenvironment, primarily influenced by cytokines and tumor-associated factors. Research has consistently linked elevated MDSC levels to unfavorable cancer prognosis and poor responses to immunotherapies.
View Article and Find Full Text PDFPeripheral blood lymphocyte immunophenotyping (TBNK) - a comparison of BD FACSCanto II and BD FACSLyric flow cytometry analysers.
Cent Eur J Immunol
March 2024
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Poland.
Introduction: Flow cytometry immunophenotyping is a common laboratory technique for evaluating lymphocyte subpopulations. Its result remains an important diagnostic tool in various medical fields. Cytometric tests are performed in many laboratories, making the comparability between different devices using the same method an important aspect.
View Article and Find Full Text PDFFast and Accurate Multiplex Identification and Quantification of Seven Genetically Modified Soybean Lines Using Six-Color Digital PCR.
Foods
November 2023
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 121, 1000 Ljubljana, Slovenia.
The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically multiplexing, offers a solution by enabling simultaneous quantification of multiple GMO targets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!