AI Article Synopsis
- Transcription factors (TFs) are essential for controlling gene-expression programs, and understanding their interactions helps clarify how they regulate embryonic stem (ES) cells.
- This study uses ChIP-seq technology to map the locations of 13 specific TFs and 2 transcription regulators involved in ES-cell biology, linking them to signaling pathways like LIF and BMP.
- The research reveals that many TFs target specific genomic regions, enhancing our understanding of the transcriptional regulatory networks that establish and maintain ES-cell identity.
Article Abstract
Transcription factors (TFs) and their specific interactions with targets are crucial for specifying gene-expression programs. To gain insights into the transcriptional regulatory networks in embryonic stem (ES) cells, we use chromatin immunoprecipitation coupled with ultra-high-throughput DNA sequencing (ChIP-seq) to map the locations of 13 sequence-specific TFs (Nanog, Oct4, STAT3, Smad1, Sox2, Zfx, c-Myc, n-Myc, Klf4, Esrrb, Tcfcp2l1, E2f1, and CTCF) and 2 transcription regulators (p300 and Suz12). These factors are known to play different roles in ES-cell biology as components of the LIF and BMP signaling pathways, self-renewal regulators, and key reprogramming factors. Our study provides insights into the integration of the signaling pathways into the ES-cell-specific transcription circuitries. Intriguingly, we find specific genomic regions extensively targeted by different TFs. Collectively, the comprehensive mapping of TF-binding sites identifies important features of the transcriptional regulatory networks that define ES-cell identity.
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| http://dx.doi.org/10.1016/j.cell.2008.04.043 | DOI Listing |
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