Recent studies of the structure of messenger RNA have demonstrated the existence of untranslated sequences of the 3' and 5' end of the messages. In addition analysis of transcription in vitro has indicated that the nucleotide sequence U6 purine may be part of a transcription termination signal in prokaryotes. Recently it has been possible to determine the sequence of extensive portions of the DNA of SV40 virus. This article reviews the analogies between certain of these sequences and sequences available from prokaryotic messengers and DNAs. Unusual structures, including blocks of AT-rich and GC-rich segment sections and symmetric regions in the DNA near the origin of DNA replication, have been demonstrated and the distribution of stretches of 6 or more deoxyadenylic acids in the DNA of SV40 is consistent with some rho for these sequences in animal cells, either as terminators of transcription or as sites where degradation of transcripts is initiated or sites related to the selective rejection or degradation of transcipts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mpo.2950020306 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line].
Mol Biol (Mosk)
December 2024
Center of Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
The low knock-in efficiency, especially in primary human cells, limits the use of the genome editing technology for therapeutic purposes, rendering it important to develop approaches for increasing the knock-in levels. In this work, the efficiencies of several approaches were studied using a model of knock-in of a construct coding for the peptide HIV fusion inhibitor MT-C34 into the human CXCR4 locus in the CEM/R5 T cell line. First, donor DNA modification was evaluated as a means to improve the efficiency of plasmid transport into the nucleus.
View Article and Find Full Text PDFPolyomavirus large T antigens: Unraveling a complex interactome.
Tumour Virus Res
December 2024
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, 15260, USA. Electronic address:
Article Synopsis
- All polyomaviruses produce a large T antigen (LT) protein that is crucial for viral DNA replication, gene regulation, and influencing cellular functions.
- Over 100 polyomaviruses exist, with 14 known to infect humans, yet most research on LT focuses on simian virus 40 (SV40) and a few others.
- This review emphasizes the differences and similarities among LT proteins of human polyomaviruses and identifies gaps in our knowledge, particularly due to the limited studies on LT proteins from various polyomavirus species.
Clusterin Deficiency Promotes Cellular Senescence in Human Astrocytes.
Mol Neurobiol
December 2024
Department of Physiology, Faculty of Science, Charles University, Prague, 128 00, Czech Republic.
Article Synopsis
- - The study focuses on the glycoprotein clusterin (CLU), which is important for cell growth and repairing DNA, and its effects on human astrocytic cell lines, showing that suppressing CLU leads to decreased cell growth and increased DNA damage response.
- - Researchers found that reducing CLU levels caused oxidative stress and triggered cellular aging (senescence) in astrocytoma cells and normal astrocytes, impacting mitochondrial function and altering energy production markers.
- - The findings highlight the crucial role of CLU in maintaining the cell cycle in astrocytes, suggesting that targeting CLU could be a promising strategy for treating gliomas (a type of brain tumor).
Sequence specificity of an essential nuclear localization sequence in Mcm3.
bioRxiv
November 2024
Biomedical Science graduate program, School of Medicine, University of California at San Diego.
Proteins with nuclear localization sequences (NLSs) are directed into the cell nucleus through interactions between the NLS and importin proteins. NLSs are generally short motifs rich in basic amino acids; however, identifying NLSs can be challenging due to the lack of a universally conserved sequence. In this study, we characterized the sequence specificity of an essential and conserved NLS in Mcm3, a subunit of the replicative DNA helicase.
View Article and Find Full Text PDFBK Virus Nephropathy After Kidney Transplantation and Its Diagnosis Using Urinary Micro RNA.
Transplant Proc
November 2024
Department of Surgery Nephrology Center, Toranomon Hospital, Tokyo Japan.
BK virus-associated nephritis (BKVAN) is an important cause of graft loss in renal transplant recipients B K viremia occurs in up to 30% of renal transplant recipients. Since the discovery of BKV in 1971, effective prophylaxis and treatment have not been established, and it is not uncommon for a transplant kidney to be lost without cure of BKVAN. BK virus infection is reactivated when cellular immunity is suppressed, which is often during the first year after kidney transplantation when cellular immunity is most suppressed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!