The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMART technology. The titers of the non-amplified library and the amplified library were 1.1 x 106 pfu/mL and 3.98 x 107 pfu/mL, respectively. The recombination rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were predicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon.
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| http://dx.doi.org/10.3724/sp.j.1005.2008.00776 | DOI Listing |
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