AI Article Synopsis
- The study aims to investigate changes in the expression of the apoptosis-related protein ARTS in lung tissues following acute pulmonary embolism (APE) in rats, focusing on how it affects cell apoptosis.
- Methods included creating a rat model of APE, collecting lung tissue samples at different times, measuring ARTS and related protein levels using techniques like RT-PCR and Western blotting, and identifying apoptotic cells through the TUNEL method.
- Results showed significant increases in ARTS mRNA and protein levels at specific time points after APE, with a notable presence in bronchial and alveolar epithelial cells, alongside a rise in apoptosis levels and a decrease in anti-apoptotic proteins compared to healthy controls.
Article Abstract
Objective: To study the expression changes in apoptosis related protein in transforming growth factors-beta signaling pathway (ARTS) in the lung tissues in a rat acute pulmonary embolism (APE) model and its effects on cell apoptosis.
Methods: A rat APE model was reproduced. Samples of lung tissues were harvested at time points of 1, 8, 24 and 48 hours after APE. Healthy rats were used as control. The changes in mRNA level of ARTS were identified by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the changes in its protein level, and also Bcl-2, Bcl-xL, XIAP and H2Ax proteins, which were related with ARTS-mediated cell apoptosis, were determined by Western blotting. Immunohistochemical method was employed to study the distribution and expression changes in ARTS in the lung tissue before and after APE. Apoptotic cells in lung tissue sections were identified by the terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) method.
Results: At different time points, the mRNA levels and the protein levels of ARTS significantly increased in the lung tissues of rats with APE at 1 hour and 48 hours (P<0.05 or P<0.01). The immunohistochemical study showed that ARTS had low expression levels and could not be detected in the normal lung tissue, but it was up-regulated obviously at 48 hours after APE, mainly expressed in the bronchial epithelium and the lung alveolar epithelium. Apoptotic cells could be observed in the lung tissue by TUNEL after APE and at the same time when the lung tissue cells exhibited lower levels of the anti-apoptotic proteins Bcl-2, Bcl-xL, and XIAP, as compared with controls. Apoptosis level (as evaluated by H2Ax, apoptotic marker staining) in the lung tissue cells was obviously raised compared with controls (P<0.05 or P<0.01).
Conclusion: The expression of ARTS is up-regulated after APE, and ARTS-mediated apoptosis plays an important role in the cell apoptosis of lung tissue.
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