AI Article Synopsis
- Advances in plasmonic nanoparticle synthesis are creating new possibilities for biosensing applications, specifically using stellated nanoparticles.
- The research focuses on detecting molecular assemblies and tracking individual epidermal growth factor receptors in live cells through sophisticated imaging techniques.
- Stellated nanoparticles offer over 15 times more efficient light depolarization compared to spheroidal nanoparticles, enabling clearer imaging with minimal background interference and sustained single-molecule sensitivity.
Article Abstract
Advances in plasmonic nanoparticle synthesis afford new opportunities for biosensing applications. Here, we apply a combination of a new type of plasmonic nanomaterial - stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio. Depolarization of linear polarized light by stellated nanoparticles is over 15-fold more efficient than similarly-sized spheroidal nanoparticles. This efficient light depolarization allows robust detection of molecules labeled with stellated nanoparticles in cross-polarized imaging where the intrinsic light scattering from cells is significantly reduced. The imaging can be carried out with single molecule sensitivity for essentially unlimited time with no signal degradation.
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| http://dx.doi.org/10.1364/oe.16.002153 | DOI Listing |
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