A reverse zymogram method for the detection of bacterial lysozyme inhibitors was developed. This method was validated by using a periplasmic protein extract of Escherichia coli containing a known inhibitor and subsequently led to the detection of a new proteinaceous hen egg white lysozyme inhibitor in Proteus mirabilis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519368 | PMC |
| http://dx.doi.org/10.1128/AEM.00219-08 | DOI Listing |
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