Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.pep.2008.04.009 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!