Cytoarchitecture of the accessory olfactory bulb in the salamander Plethodon shermani.

Plethodontid terrestrial salamanders are emerging models in the study of the evolution of chemical communication in vertebrates. Their vomeronasal system is well defined. It comprises sensory neurons in the epithelium of the vomeronasal organ, whose axons form the vomeronasal nerve projecting to the accessory olfactory bulb (AOB), which in turn projects to the vomeronasal amygdala through the accessory olfactory tract. A detailed description of the cellular elements of the urodele AOB is lacking. Neuronal morphology in the AOB was studied by means of biocytin intracellular injections and retrograde tract tracing in the salamander Plethodon shermani. The AOB exhibits the characteristic lamination of olfactory bulbs, except that it displays a mixed periglomerular and mitral somata layer superficially. Mitral cells are the only AOB neurons projecting to the vomeronasal amygdala. Each mitral cell sends multiple axonal branches, generally through both dorsal and ventral portions of the accessory olfactory tract. Some mitral cells additionally send axon collaterals in the white matter immediately ventral to the AOB. AOB interneurons are divided into superficial periglomerular and deep granule cells, each category exhibiting morphological variety. Some neurons in the granule cell layer of the AOB or the region ventral to the AOB have dendritic trees that cover both regions. The present study is the first to highlight the full anatomical extent of single AOB neurons and surprisingly suggests that the ventrolateral telencephalon found below the AOB is part of the salamander vomeronasal system.