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Thromboelastometry-Based Profiling of Haemostatic Alterations in Neonatal Sepsis by Causative Pathogens.
Antibiotics (Basel)
January 2025
Laboratory of Haematology and Blood Bank Unit, "Attikon" Hospital, National and Kapodistrian University of Athens Medical School, 12462 Athens, Greece.
: Neonatal sepsis is a serious condition with high mortality, especially in premature and low-birth-weight neonates. This study aims to examine whether the haemostatic profile of neonates with sepsis defers depending on the type of bacteria (Gram-positive or Gram-negative), by using the method of Rotational Thromboelastometry (ROTEM). : This single-centre prospective cohort study was conducted on 128 neonates with sepsis, including 95 cases caused by Gram-negative pathogens and 33 cases caused by Gram-positive bacteria.
View Article and Find Full Text PDFAn animal model of severe acute respiratory distress syndrome for translational research.
Lab Anim Res
January 2025
Department of Anatomy and Cell Biology, School of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Li-Nung Street, Taipei, Taiwan, ROC.
Background: Despite the fact that an increasing number of studies have focused on developing therapies for acute lung injury, managing acute respiratory distress syndrome (ARDS) remains a challenge in intensive care medicine. Whether the pathology of animal models with acute lung injury in prior studies differed from clinical symptoms of ARDS, resulting in questionable management for human ARDS. To evaluate precisely the therapeutic effect of transplanted stem cells or medications on acute lung injury, we developed an animal model of severe ARDS with lower lung function, capable of keeping the experimental animals survive with consistent reproducibility.
View Article and Find Full Text PDF[Early efficacy of local tranexamic acid in reducing postoperative swelling in hallux valgus surgery].
Beijing Da Xue Xue Bao Yi Xue Ban
February 2025
Department of Foot and Ankle Surgery, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100035, China.
Objective: To evaluate the early efficacy of local application of tranexamic acid on the osteotomy surface during hallux valgus surgery in reducing postoperative occult blood loss and thus postoperative swelling.
Methods: The data of 40 cases with hallux valgus osteotomy admitted to the Department of Foot and Ankle Surgery of Jishuitan Hospital from July 11, 2022 to October 8, 2022, including 5 males and 35 females were retrospectively analyzed. According to the inclusion and exclusion criteria, 32 cases were finally divided into 16 cases in the observation group (application of tranexamic acid) and 16 cases in the control group (no application of tranexamic acid).
The Role of Rotational Thromboelastometry in Early Detection of the Hemostatic Derangements in Neonates with Systemic Candida Infection.
J Fungi (Basel)
December 2024
Laboratory of Haematology and Blood Bank Unit, "Attikon" Hospital, National and Kapodistrian University of Athens Medical School, 12462 Athens, Greece.
Background: Systemic infection (SCI) is the third most common cause of late-onset sepsis in Neonatal Intensive Care Units (NICU). While platelet involvement in fungal infections has been extensively studied, evaluation of the hemostatic mechanism in Candida infections, especially in neonates, has not been widely investigated. The aim of the current study was to evaluate the hemostatic profile of neonates with SCI through rotational thromboelastometry (ROTEM), a laboratory method that assesses the viscoelastic properties of blood.
View Article and Find Full Text PDFLabel-free imaging flow cytometry for cell classification based directly on multiple off-axis holographic projections.
J Biomed Opt
January 2025
Tel Aviv University, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv, Israel.
Significance: Imaging flow cytometry allows highly informative multi-point cell analysis for biological assays and medical diagnosis. Rapid processing of the imaged cells during flow allows real-time classification and sorting of the cells. Off-axis holography enables imaging flow cytometry without chemical cell staining but requires digital processing to the optical path delay profile for each frame before the cells can be classified, which slows down the overall processing throughput.
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