The fluorescence lifetime image of HeLa cells expressing an enhanced green fluorescent protein (EGFP)-fusion protein changes under stress, which allows noninvasive determination of the status of individual cells.
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http://dx.doi.org/10.1039/b805032e | DOI Listing |
Photosynth Res
January 2025
State Key Laboratory of Forage Breeding-by-Design and Utilization, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Maize (Zea mays L.) performs highly efficient C photosynthesis by dividing photosynthetic metabolism between mesophyll and bundle sheath cells. In vivo physiological measurements are indispensable for C photosynthesis research as photosynthetic activities are easily interrupted by leaf section or cell isolation.
View Article and Find Full Text PDFAm J Respir Cell Mol Biol
January 2025
University of Colorado Denver School of Medicine, Aurora, Colorado, United States;
Whether early life acetaminophen (APAP) exposures injure the developing lung is controversial. We sought to correlate murine pulmonary developmental expression profiles of to susceptibility to APAP exposure. P14 C57BL/6 mice were exposed to APAP (140 mg/kg x 1, IP) and assessed for evidence of a histologic, metabolic, functional, and/or transcriptional pulmonary response.
View Article and Find Full Text PDFPhotochem Photobiol
January 2025
Departamento de Bioquímica, Facultad de Ciencias Naturales, Exactas y Tecnología, Universidad de Panamá, Panamá, Republic of Panama.
Toluidine blue O (TBO) is a type I-type II photosensitizer that has shown good efficacy and selectivity in antimicrobial and anticancer photodynamic therapy applications. However, its complex photochemistry with multiple photoproducts hinders its application as a photosensitizer. We have previously described the mechanism for photooxidative demethylation of TBO which in acetonitrile yields two main products: demethylated-TBO (d-TBO) and double-demethylated-TBO (dd-TBO).
View Article and Find Full Text PDFBull Environ Contam Toxicol
January 2025
School of Mathematics and Physics, Xi'an Jiaotong-Liverpool University, Suzhou, Jiangsu, 215123, China.
This study focuses on the composition and sources of dissolved organic matter (DOM) in the Fancun Reservoir, located in Ningguo City, Anhui Province, China. The investigation was conducted by analyzing the spectral characteristics of DOM using UV-Vis absorption spectra and fluorescence spectroscopy. The humic substances were dominated by fulvic acid, with an average DOM concentration of 30.
View Article and Find Full Text PDFSoft Matter
January 2025
INSERM (French National Institute of Health and Medical Research), UMR 1260, Regenerative Nanomedicine (RNM), FMTS, Université de Strasbourg, F-67000 Strasbourg, France.
Characterization of nanoscale formulations is a continuous challenge. Size, morphology and surface properties are the most common characterizations. However, physicochemical properties inside the nanoparticles, like viscosity, cannot be directly measured.
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