AI Article Synopsis
- Maintaining nonmalignant cells in sarcoma cell cultures helps mimic the original tumor environment, providing necessary support for tumor cell growth.
- These nonmalignant cells contribute paracrine factors and help protect tumor cells from undergoing apoptosis (cell death).
- In contrast, cultures that eliminate these supporting cells rely solely on autocrine signals from the tumor cells themselves, which may not accurately represent the native tumor behavior.
Article Abstract
Human sarcoma cell populations maintained in culture reflect to the native tumor cells better if the culture retains those nonmalignant cells that comprised the tumor's microenvironment in vivo [Hu M, et al. Characterization of 11 human sarcoma cell strains. Cancer 2002; 95: 1569-76] and thus provide paracrine growth factors and protection from apoptotic death to the tumor cells. Whereas sarcoma cell cultures obtained through meticulous efforts aimed at the elimination of all non-malignant cells of the tumor's original microenvironment consist of subpopulations of tumor cells growing exclusively with the support of their own autocrine growth loops [Sinkovics JG, et al. Growth of human tumor cells in established cultures. In: Busch H, ed. Methods in Cancer Research. Vol 14. New York: Academic Press, 1978; 243-323].
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2395523 | PMC |
| http://dx.doi.org/10.1080/13577140310001607301 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!