A new method for on-plate protein digestion and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry analysis is proposed involving an automated one-step sample separation using nanoflow HPLC followed by nanoliter fraction collection and on-plate digestion with trypsin. This procedure uses a commercial automatic nanoliter fraction collection system for on-line spotting of the eluent onto a MALDI target. After protein digestion, the reaction is stopped by the addition of acidified matrix using the same automated system. Collected spots are subsequently analyzed using a MALDI tandem time-of-flight (TOF/TOF) mass spectrometer for protein sequencing and identification.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2805255 | PMC |
| http://dx.doi.org/10.1016/j.ab.2008.05.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gluten-Free Bread Enriched with Artichoke Leaf Extract In Vitro Exerted Antioxidant and Anti-Inflammatory Properties.
Antioxidants (Basel)
April 2023
Department of Soil, Plant and Food Science (DiSSPA), University of Bari Aldo Moro, 70126 Bari, Italy.
Due to its high nutritional value and broad beneficial effects, the artichoke plant ( L.) is an excellent healthy food candidate. Additionally, the artichoke by-products are usually discarded even though they still contain a huge concentration of dietary fibers, phenolic acids, and other micronutrients.
View Article and Find Full Text PDFReady-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome.
Microbiol Spectr
February 2023
Division of Food Microbiology and Bioprocesses, Department of Food, Environmental, and Nutritional Sciences, Università degli Studi di Milano, Milan, Italy.
Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases. It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure. In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
View Article and Find Full Text PDFKnockdown of Long Noncoding RNA LINC00240 Inhibits Esophageal Cancer Progression by Regulating miR-26a-5p.
Contrast Media Mol Imaging
October 2022
Department of Thoracic Surgery, Tumor Hospital Affiliated to Nantong University, Nantong 226000, China.
Background: Esophageal cancer is the most prevalent digestive system tumor. Due to a lack of characteristic symptoms and early diagnosis, a confirmed esophageal cancer is typically detected at a progressively harmful stage. Therefore, it is critical to investigate the molecular mechanisms governing the formation and progression of esophageal cancer in order to identify new treatment targets for esophageal cancer early detection.
View Article and Find Full Text PDFPhosphorus limitation does not drive loss of bony lateral plates in freshwater stickleback (Gasterosteus aculeatus).
Evolution
September 2020
Institute of Ecology and Evolution, University of Bern, Bern, CH-3012, Switzerland.
Connecting the selective forces that drive the evolution of phenotypes to their underlying genotypes is key to understanding adaptation, but such connections are rarely tested experimentally. Threespine stickleback (Gasterosteus aculeatus) are a powerful model for such tests because genotypes that underlie putatively adaptive traits have been identified. For example, a regulatory mutation in the Ectodysplasin (Eda) gene causes a reduction in the number of bony armor plates, which occurs rapidly and repeatedly when marine sticklebacks invade freshwater.
View Article and Find Full Text PDFSurgical Stress Increases Circulating Low-Density Neutrophils Which May Promote Tumor Recurrence.
J Surg Res
February 2020
Department of Gastrointestinal Surgery, Jichi Medical University, Shimotsuke City, Tochigi, Japan. Electronic address:
Background: Low-density neutrophils (LDN) have been shown to be increased in peripheral blood in patients with various diseases and closely related to immune-mediated pathology. However, the frequency and function of LDN in circulating blood of the patients following abdominal surgery have not been well understood.
Methods: LDN were determined by CD66b(+) cells, which were copurified with mononuclear cells by density gradient preparations of peripheral blood of surgical patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!