Effect of the alkaline treatment on the ultrastructure of C-type starch granules.

Biomacromolecules

Programa de Pos-Graduacao em Engenharia Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Published: July 2008

The effect of alkaline treatment on the ultrastructure of C-type starch granules was investigated during the alkaline extraction of Araucaria angustifolia (pinhao) starch. The efficiency in protein removal was evaluated using intrinsic fluorescence and Kjeldahl's method. In parallel, morphological changes of starch granules were observed using scanning electron microscopy and atomic force microscopy. The starch crystallinity was monitored by wide-angle X-ray scattering and the lamellar structure was studied by small-angle X-ray scattering (SAXS). The paracrystalline model was employed to interpret the SAXS curves. It was found that the granular organization was significantly altered when alkaline solutions were used during the extraction. A partial degradation of B-type allomorph of starch and a significant compression of semicrystalline growth rings were observed.

Download full-text PDF

Source
http://dx.doi.org/10.1021/bm800143wDOI Listing

Publication Analysis

Top Keywords

starch granules
12
alkaline treatment
8
treatment ultrastructure
8
ultrastructure c-type
8
c-type starch
8
x-ray scattering
8
starch
6
alkaline
4
granules alkaline
4
granules investigated
4

Similar Publications

We investigated the effects of exogenous abscisic acid (ABA) on grain filling, starch accumulation, and endogenous hormones in maize (both the heat-tolerant maize variety Zhengdan 958 (ZD958) and the heat-sensitive variety Xianyu 335 (XY335)) under early post-anthesis high temperature stress by simulating high temperature stress for a period of 6 to 12 days post-anthesis in 2022 and 2023. There were three treatments: spraying water at ambient temperature as the control, spraying water at high temperature, and spraying ABA at high temperature. The results showed that early post-anthesis high temperature stress resulted in a significant reduction in grain weight and yield in maize, with XY335 showing a greater reduction than ZD958.

View Article and Find Full Text PDF

Deciphering heat wave effects on wheat grain: focusing on the starch fraction.

Front Plant Sci

December 2024

LEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal.

Wheat is an essential staple food, and its production and grain quality are affected by extreme temperature events. These effects are even more relevant considering the increasing food demand for a growing world population and the predicted augmented frequency of heat waves. This study investigated the impact of simulated heat wave (HW) conditions imposed during grain filling on starch granule characteristics, endosperm ultrastructure, and transcriptomic modulation of genes involved in starch synthesis and degradation.

View Article and Find Full Text PDF
Article Synopsis
  • * Overexpression of MaFLA27 led to increased expression of genes involved in cell wall components and modification, contributing to thicker cell walls and higher levels of cellulose, lignin, and certain pectins in plants.
  • * In contrast to wild-type plants, MaFLA27-overexpressing plants showed lower levels of pectin methyl-esterification and reduced reactive oxygen species after cold exposure, indicating a potential mechanism for improved cold tolerance linked to cell wall modifications.
View Article and Find Full Text PDF

Gem-like starch granules engineered with different crystalline starches.

Food Chem

December 2024

School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address:

The retrogradation property endows starch molecules with the unique ability to self-assemble crystals after encountering damage, which can restore the internal double helix structure to a limited extent. We devise a simple set of steps to grow perfect crystals of uniform size with different crystal systems in a given growth environment by first customizing the crystal seeds through enzymatic modification, and then exploiting the spontaneous recrystallization of starch. Optical microscopy confirmed the formation of gem-like starch granules (GSGs) in starches with 24.

View Article and Find Full Text PDF

Rice, a staple in diets, undergoes digestion post-consumption, often triggering a swift surge in blood sugar among diabetics, intensifying their health burden. Notably, resistant starch (RS) emerges as a potent ally in fostering satiety and mitigating metabolic syndrome in diabetes. The SBEIIb gene, a key orchestrator of starch branching enzymes, plays a pivotal role in starch synthesis, and its genetic alteration can dramatically boost RS content in rice.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!