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Electroporation as an efficient method of gene transfer. | LitMetric
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Electroporation as an efficient method of gene transfer.

Noritaka Odani Kodai Ito Harukazu Nakamura

Dev Growth Differ

Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.

Published: August 2008

Gene transfer by electroporation is an indispensable method for the study of developmental biology, especially for the study using chick embryos. Here we briefly review the principles of the method, and its application to chick embryos. Methods of transient misexpression and long-term misexpression by retrovirus vector or transposon system, and knockdown by small interference RNA are reviewed.

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 Source
http://dx.doi.org/10.1111/j.1440-169X.2008.01037.xDOI Listing

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