RNA methylation, which is a form of posttranscriptional modification, is catalyzed by S-adenosyl-L-methionone-dependent RNA methyltransterases (RNA MTases). We have identified a novel silkworm gene, BmRNAMTase, containing a 369-bp open reading frame that encodes a putative protein containing 122 amino acid residues and having a molecular weight of 13.88 kd. We expressed a recombinant His-tagged BmRNAMTase in E. coli BL21 (DE3), purified the fusion protein by metal-chelation affinity chromatography, and injected a New Zealand rabbit with the purified protein to generate anti-BmRNAMTase polyclonal antibodies. Immunohistochemistry revealed that BmRNAMTase is abundant in the cytoplasm of Bm5 cells. In addition, using RNA interference to reduce the intracellular activity and content of BmRNAMTase, we determined that this cytoplasmic RNA methyltransferase may be involved in preventing cell death in the silkworm.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396236 | PMC |
| http://dx.doi.org/10.1155/2008/571023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exogenous dsRNA triggers sequence-specific RNAi and fungal stress responses to control Magnaporthe oryzae in Brachypodium distachyon.
Commun Biol
January 2025
Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.
In vertebrates and plants, dsRNA plays crucial roles as PAMP and as a mediator of RNAi. How higher fungi respond to dsRNA is not known. We demonstrate that Magnaporthe oryzae (Mo), a globally significant crop pathogen, internalizes dsRNA across a broad size range of 21 to about 3000 bp.
View Article and Find Full Text PDFIdentification and functional analyses of the CmdsRNase5 and CmdsRNase6 genes in rice leaffolder Cnaphalocrocis medinalis.
Int J Biol Macromol
January 2025
Guizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China. Electronic address:
RNA interference (RNAi) is a promising method for pest control; however, some studies have showed that the degradation of double-stranded RNA (dsRNA) by dsRNA-degrading nucleases (dsRNases) is one of the factors that may reduce RNAi efficiency in lepidopteran insects. In this study, we cloned two dsRNase genes named CmdsRNase5 and CmdsRNase6 from rice leaffolder Cnaphalocrocis medinalis, a notorious insect pest of rice. Open reading frames (ORFs) of CmdsRNase5 and CmdsRNase6 are 1317 and 1185 bp in length, encoding 438 and 394 amino acids, respectively.
View Article and Find Full Text PDFDengue virus is particularly sensitive to interference with long-chain fatty acid elongation and desaturation.
J Biol Chem
January 2025
Institute of Virology, Philipps University Marburg, Marburg, Germany. Electronic address:
Orthoflaviviruses are emerging arthropod-borne pathogens whose replication cycle is tightly linked to host lipid metabolism. Previous lipidomic studies demonstrated that infection with the closely related hepatitis C virus (HCV) changes the fatty acid (FA) profile of several lipid classes. Lipids in HCV-infected cells had more very long-chain and desaturated FAs and viral replication relied on functional FA elongation and desaturation.
View Article and Find Full Text PDFRegulation of tick attachment and rapid engorgement via dopamine receptors in the Asian longhorned tick Haemaphysalis longicornis.
Insect Biochem Mol Biol
January 2025
Department of Vector Entomology, Kyungpook National University, Sangju, Republic of Korea; Research Institute of Invertebrate Vector, Kyungpook National University, Sangju, Republic of Korea. Electronic address:
Dopamine plays multifaceted roles in the physiology of insects and ticks, acting as a key neurotransmitter and modulator of various biological processes. In ticks, it plays a particularly important role in regulating salivary gland function, which is essential for successful tick feeding on hosts. Salivary secretion in ticks is orchestrated by the collection of saliva in the acinar lumen mediated by the dopamine receptor (D1) and the expulsion of collected saliva into the salivary duct mediated by the invertebrate specific D1-like dopamine receptor (InvD1L).
View Article and Find Full Text PDFRNAi-based biocontrol for crops: a revised expectation for a non-recent technology.
Planta
January 2025
School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia.
The exogenous application of RNAi technology offers new promises for crops improvement. Cell-based or synthetically produced strands are economical, non-transgenic and could induce the same responses. The substantial population growth demands novel strategies to produce crops without further damaging the environment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!