The viability of the toluate moiety as a radical precursor has been examined by studying deoxygenation and cyclization reactions.
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http://dx.doi.org/10.1021/ol800944p | DOI Listing |
PLoS One
May 2016
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, United States of America; Centre of Molecular Inflammation Research, Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Central Norway Regional Health Authority, Stjørdal, Norway.
Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in Mycobacterium smegmatis and Mycobacterium tuberculosis, the causative agent of human tuberculosis. By making simple changes to a Gram-negative broad-host-range XylS/Pm-regulated gene expression vector, we prove that it is possible to adapt this well-studied expression system to non-Gram-negative species.
View Article and Find Full Text PDFPLoS One
October 2017
Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly used in protocols for analysis of mRNA stability. Here we show that such treatment can be effectively replaced by a simple, non-invasive method based on removal of the relevant transcriptional inducers and that the mRNA decay can then be followed by qRT-PCR.
View Article and Find Full Text PDFJ Phys Chem B
September 2010
State Key Lab of Metastable Materials Science and Technology and College of Materials Science and Engineering, Yanshan University, Qinhuangdao, Hebei, 066004 China.
The glass transition and glass-forming ability in a binary eutectic system of methyl o-toluate (MOT) versus methyl p-toluate (MPT) are studied across the whole composition range. The phase diagram is constructed to explore the best glass-forming composition as the characteristic temperatures of the glass transition, crystallization, eutectic, and liquidus are determined. The best vitrification region is found to locate between the eutectic and the midpoint compositions of the eutectic line, indicating a remarkable deviation from the eutectic composition.
View Article and Find Full Text PDFChem Commun (Camb)
January 2009
Département de Chimie, Bâtiment Lavoisier, Université catholique de Louvain, B-1348, Louvain-la-Neuve, Belgium.
The electrolysis of toluate esters leads smoothly to the formation of the radical of the alkyl fragment. This property has been used to develop a new electrochemical deoxygenation reaction.
View Article and Find Full Text PDFOrg Lett
July 2008
Département de Chimie, Bâtiment Lavoisier, Université catholique de Louvain, Place Louis Pasteur, 1, B-1348 Louvain-la-Neuve, Belgium.
The viability of the toluate moiety as a radical precursor has been examined by studying deoxygenation and cyclization reactions.
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