Precursor lymphocytes, obtained from human tissue on bovine serum albumin (BSA) gradients followed by removal of sheep erythrocyte (E)-reactive thymocytes on Ficoll, are the predominant lymphoid subpopulation found in young fetal thymuses. This subpopulation shows a progressive decline in relative numbers during fetal life, accompanied by a rise in both the relative and absolute numbers of E-reactive thymocytes. Precursor thymocytes differentiate in vitro to E-reactive cells resembling T cells in their morphology, sedimentation on BSA gradients, and response to phytohemagglutinin. Agents tending to increase intracellular concentrations of cyclic-AMP accelerate the rate of differentiation of precursor cells to lymphocytes which closely resemble T cells.
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