Purpose: To examine antiepileptogenic and antiictogenic potential of retigabine (RTG) under conditions of rapid kindling epileptogenesis during different stages of development.
Methods: The experiments were performed in postnatal day 14 (P14), P21, and P35 male Wistar rats. After stereotaxic implantation of hippocampal stimulating and recording electrodes, the effects of RTG on baseline afterdischarge (AD) properties were studied. Next, the animals underwent rapid kindling (sixty 10 s trains, bipolar 20 Hz square wave pulses delivered every 5 min). The progression of seizures (kindling acquisition), and responses to test stimulations after kindling (retention) were compared between RTG and vehicle-treated rats. Additionally, the effects of RTG on the severity of seizures in previously kindled animals were examined.
Results: When administered intraperitoneally in doses that induced only mild, or no motor deficits, RTG significantly dampened brain excitability, evident as the increase of AD threshold and shortening of AD duration. During kindling, RTG delayed the development of focal seizures in P14 rats, and prevented the occurrence of full limbic seizures at all three ages. At P14 and P21, but not at P35, pretreatment with RTG prevented the establishment of kindling-induced enhanced seizure susceptibility. Administration of RTG to kindled animals decreased the severity of seizures induced by test stimulation. The effect was most prominent at P14.
Discussion: RTG exerted both antiepileptogenic and antiictogenic effects under conditions of rapid kindling model. These effects were apparent during postneonatal, early childhood, and adolescent stages of development.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577127 | PMC |
| http://dx.doi.org/10.1111/j.1528-1167.2008.01674.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
On-Demand Seizures Facilitate Rapid Screening of Therapeutics for Epilepsy.
bioRxiv
October 2024
Department of Neurology, Perelman School of Medicine, University of Pennsylvania; Philadelphia, PA, 19104, USA.
Animal models of epilepsy are critical in drug development and therapeutic testing, but dominant methods for pharmaceutical evaluation face a tradeoff between higher throughput and etiological relevance. For example, in temporal lobe epilepsy, a type of epilepsy where seizures originate from limbic structures like the hippocampus, the main screening models are either based on acutely induced seizures in wild type, naïve animals or spontaneous seizures in chronically epileptic animals. Both types have their disadvantages - the acute convulsant or kindling induced seizures do not account for the myriad neuropathological changes in the diseased, epileptic brains, and spontaneous behavioral seizures are sparse in the chronically epileptic models, making it time-intensive to sufficiently power experiments.
View Article and Find Full Text PDFKindling Models of Epileptogenesis for Developing Disease-Modifying Drugs for Epilepsy.
Curr Protoc
October 2024
Department of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M University Health Science Center, Bryan, Texas.
Article Synopsis
- Kindling models are important animal models for studying epilepsy, characterized by the gradual progression from brief focal seizures to generalized tonic-clonic seizures through sub-threshold stimulation.
- These models utilize techniques like implanted electrodes and chemoconvulsants to induce seizures, allowing researchers to test new antiseizure medications with high predictive validity.
- The article offers detailed protocols for different kindling methods, along with insights into using pharmacological agents and genetically modified mice to better understand seizure dynamics and potential treatments for epilepsy.*
Effective reduction in seizure severity and prevention of a fatty liver by a novel low ratio ketogenic diet composition in the rapid kindling rat model of epileptogenesis.
Exp Neurol
September 2024
Amsterdam UMC location University of Amsterdam, Dept of (Neuro)pathology, Amsterdam Neuroscience, Meibergdreef 9, Amsterdam, the Netherlands; Stichting Epilepsie Instellingen Nederland (SEIN), Heemstede, the Netherlands.
Drug-resistant epilepsy patients may benefit from non-pharmacological therapies, such as the ketogenic diet (KD). However, its high fat content poses compliance challenges and metabolic risks. To mitigate this, we developed a novel KD composition with less fat and additional nutrients (citrate, nicotinamide riboside, and omega-3 fatty acids) for ketone-independent neuroprotection.
View Article and Find Full Text PDFNanozyme as detector and remediator to environmental pollutants: between current situation and future prospective.
Environ Sci Pollut Res Int
January 2024
Chemistry/Biochemistry Sector, Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
The term "nanozyme" refers to a nanomaterial possessing enzymatic capabilities, and in recent years, the field of nanozymes has experienced rapid advancement. Nanozymes offer distinct advantages over natural enzymes, including ease of production, cost-effectiveness, prolonged storage capabilities, and exceptional environmental stability. In this review, we provide a concise overview of various common applications of nanozymes, encompassing the detection and removal of pollutants such as pathogens, toxic ions, pesticides, phenols, organic contaminants, air pollution, and antibiotic residues.
View Article and Find Full Text PDFCompositionally engineered Cd-Mo-Se alloyed QDs toward photocatalytic HO production and Cr(VI) reduction with a detailed mechanism and influencing parameters.
Dalton Trans
November 2023
Centre for Nanoscience and Nanotechnology, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar-751030, Odisha, India.
With the exceptional advantages of safety, greenness, and low cost, photocatalytic HO generation has kindled a wonderful spark, although being severely hampered by the terrible photoinduced exciton recombination, migration, and surface decomposition. Here, employing reflux method, the Cd-Mo-Se quantum dots of varying molar ratios of Cd and Mo were synthesized using thioglycolic acid as the capping ligand to regulate their growth. This type of metal alloying promotes rapid charge migration, improves light harvesting, and reduces the rate of charge recombination.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!