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Photometric method for dual targeting of surface and surface-associated proteins on extracellular vesicles in the multiparametric test.
Front Mol Biosci
October 2022
Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
Extracellular vesicles (EVs) have become a topic of interest within the field of diagnostic biomarkers; however, recent developments in the study of EVs have increased the need for simpler but still comprehensive methods for characterization. Here, we describe how to simultaneously measure several surface or surface-associated proteins on EVs using a multiparametric microarray-based analysis termed Extracellular Vesicle Array (EV Array), which is developed to catch and phenotypically characterize small EVs. Previously, this analysis has been limited to measuring only one fluorescent signal per analysis.
View Article and Find Full Text PDF[Leishmaniasis in Ecuador. 5. Leishmaniasis and anthropization on the Pacific coast].
Ann Soc Belg Med Trop
March 1994
ORSTOM, Paris, France.
We have evaluated the impact of anthropization of the forest on the incidence of leishmaniasis, due to Leishmania panamensis, in three coastal study areas, Corriente Grande (primary forest), Paraiso Escondido and La Tablada (secondary forest). The situation of isolated dwellings, in deforested areas, has also been analysed in the last two stations. In each station, the study of the density of anthropophilic sand flies, specially Lutzomyia trapidoi, has been conducted in the domestic environment, coffee plantations and undergrowth.
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