A method for the purification of epithelial cells from the three anatomical regions of the rat epididymis (corpus, caput and cauda) is described. An enzymic digestion followed by sedimentation of crude cell suspension on discontinuous Percoll gradient yielded quite pure active epithelial cell population as judged by morphological and functional studies. Electron microscopy analysis showed that cells from bands corresponding to densities 1.055 and 1.06 g/ml the gradient preserved a morphology compatible with their epithelial origin and their absorptive and secretory functions. Moreover, they stained positively with anticytokeratin antibody (95-97%) and were negative for antidesmin antibody. They selectively bound L-carnitine through a time-dependent and saturable system and differences in the rate of binding were apparent according to the three anatomical regions of the epididymis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1530/jrf.0.0910617 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selenium triggers AMPK-mTOR pathway to modulate autophagy related to oxidative stress of sheep Leydig cells.
Reprod Biol
November 2024
College of Animal Science, Shanxi Agricultural University, Taigu 030801, PR China; Laboratory of Animal Reproductive biotechnology, Shanxi Agricultural University, Taigu 030801, PR China. Electronic address:
The objective of this study was to investigate the effect of oxidative stress induced by excessive Se on autophagy of sheep Leydig cells and its underlying mechanism. Leydig cells isolated from the testis of 8-month-old sheep were purified using a discontinuous Percoll density gradient. Cells were divided into four treatment groups (0, 2.
View Article and Find Full Text PDFThe Miniaturized Isolation of Neutrophil Granules (MING) method allowed a deep proteome mapping of human neutrophil granules.
J Leukoc Biol
October 2024
Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
Neutrophils are the innate immune system's first line of defense, and their storage organelles are essential to their function. The storage organelles are divided into three different granule types named azurophilic, specific, and gelatinase granules, besides a fourth component called secretory vesicles. The isolation of neutrophil's granules is challenging, and the existing procedures rely on large sample volumes, about 400 mL of peripheral blood, precluding the use of multiple biological and technical replicates.
View Article and Find Full Text PDFBacteria associated with the Symbiodiniaceae's phycosphere.
iScience
April 2024
Red Sea Research Center, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955, Kingdom of Saudi Arabia.
Symbiotic interactions between Symbiodiniaceae and bacteria are still poorly explored, especially those . Here, we adapted a technique that allows for the enrichment of intact and metabolically active Symbiodiniaceae cells (SC) and their associated bacteria from the tissue of the model coral , using a discontinuous gradient of solution of isotonic Percoll (SIP). The SC were concentrated in the 50% SIP fraction, as determined by microscopy.
View Article and Find Full Text PDFComparative investigation of different chemicals on the germination of two aquatic microsporidia.
J Invertebr Pathol
November 2023
Modeling in Health Research Center, Shahrekord University of Medical Sciences, Shahrekord, Islamic Republic of Iran. Electronic address:
Microsporidia are eukaryotic obligatory intracellular parasites that infect a wide range of vertebrates and invertebrate hosts. Spores infect target cells of the host by transferring their sporoplasm through a distinctive polar tube. This study investigated how selected chemicals influence the germination of two newly discovered microsporidia species from central-western Iran.
View Article and Find Full Text PDFCharacterization and enrichment of spermatogonial stem cells of common carp (Cyprinus carpio).
Theriogenology
January 2024
Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, 01049-010, Brazil. Electronic address:
Spermatogenesis is a systematically organized process that ensures uninterrupted sperm production in which the spermatogonial stem cells (SSCs) play a crucial role. However, the existing absence of teleost-specific molecular markers for SSCs presents a notable challenge. Herein we characterized phenotypically the spermatogonial stem cells using specific molecular markers and transmission electron microscopy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!