AI Article Synopsis
- STAT5A and STAT5B are closely related proteins involved in gene regulation, sharing 91% similarity in their amino acid sequences yet performing distinct functions.
- Researchers conducted experiments to knock down these proteins in cells, which helped identify new target genes activated by each STAT5 variant.
- Some of these identified target genes are found to be overactive in various human cancers, indicating their potential role as oncogenes in cancers linked to STAT5.
Article Abstract
STAT5A and STAT5B proteins belong to the family of signal transducers and activators of transcription. They are encoded by two separate genes with 91% identity in their amino acid sequences. Despite their high degree of conservation, STAT5A and STAT5B exert non-redundant functions, resulting at least in part from differences in target gene activation. To better characterize the differential contribution of STAT5A and STAT5B in gene regulation, we performed single or double knockdown of STAT5A and STAT5B using small interfering RNA. Subsequent gene expression profiling and RT-qPCR analyses of IL-3-stimulated Ba/F3-beta cells led to the identification of putative novel STAT5 target genes. Chromatin immunoprecipitation assays analyzing the corresponding gene loci identified unusual STAT5 binding sites compared to conventional STAT5 responsive elements. Some of the STAT5 targets identified are upregulated in several human cancers, suggesting that they might represent potential oncogenes in STAT5-associated malignancies.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2441806 | PMC |
| http://dx.doi.org/10.1093/nar/gkn271 | DOI Listing |
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