AI Article Synopsis
- Photodynamic therapy (PDT) utilizes visible light to activate photosensitizers, targeting cell membranes for cancer treatment.
- This study specifically examined the effects of chloroaluminum phthalocyanine liposomal on HeLa cells, focusing on their mitochondria, cytoskeleton, and endoplasmic reticulum.
- Results showed that PDT led to significant changes in mitochondrial membrane potential and disrupted the distribution of cytoskeletal components and the endoplasmic reticulum, indicating substantial photodamage to these cellular structures.
Article Abstract
Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotoxicity in specific cellular targets of a photosensitizer used in PDT in vitro. The photosensitizing effects of chloroaluminum phthalocyanine liposomal were studied on the mitochondria, cytoskeleton and endoplasmic reticulum of HeLa cells. Cells were irradiated with a diode laser working at 670 nm, energy density of 4.5 J/cm2 and power density of 45 mW/cm2. Fluorescence microscopic analysis of the mitochondria showed changes in membrane potential. After PDT treatment, the cytoskeleton and endoplasmic reticulum presented basic alterations in distribution. The combined effect of AlPHCl liposomal and red light in the HeLa cell line induced photodamage to the mitochondria, endoplasmic reticulum and actin filaments in the cytoskeleton.
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| http://dx.doi.org/10.1016/j.cellbi.2008.04.005 | DOI Listing |
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