Initiation of warfarin therapy is associated with bleeding owing to its narrow therapeutic window and unpredictable therapeutic dose. Pharmacogenetic-based dosing algorithms can improve accuracy of initial warfarin dosing but require rapid genotyping for cytochrome P-450 2C9 (CYP2C9) *2 and *3 single nucleotide polymorphisms (SNPs) and a vitamin K epoxide reductase (VKORC1) SNP. We evaluated 4 commercial systems: INFINITI analyzer (AutoGenomics, Carlsbad, CA), Invader assay (Third Wave Technologies, Madison, WI), Tag-It Mutation Detection assay (Luminex Molecular Diagnostics, formerly Tm Bioscience, Toronto, Canada), and Pyrosequencing (Biotage, Uppsala, Sweden). We genotyped 112 DNA samples and resolved any discrepancies with bidirectional sequencing. The INFINITI analyzer was 100% accurate for all SNPs and required 8 hours. Invader and Tag-It were 100% accurate for CYP2C9 SNPs, 99% accurate for VKORC1 -1639/3673 SNP, and required 3 hours and 8 hours, respectively. Pyrosequencing was 99% accurate for CYP2C9 *2, 100% accurate for CYP2C9 *3, and 100% accurate for VKORC1 and required 4 hours. Current commercial platforms provide accurate and rapid genotypes for pharmacogenetic dosing during initiation of warfarin therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1309/1E34UAPR06PJ6HML | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reliability, validity, responsiveness and minimal important changes of common clinical standing balance tests in individuals with knee osteoarthritis.
Physiother Theory Pract
January 2025
Department of Orthopaedics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Introduction: Standing balance is essential for physical functioning. Therefore, improving balance control is a key priority in the management of knee osteoarthritis (OA), underscoring the importance of accurately assessing standing balance.
Purpose: To assess reliability, construct validity and responsiveness of common clinical balance tests, including Step Test, Single-Leg Stance Test, and Functional Reach Test, in patients with knee OA.
'The Stegosaurus Appearance' on ultrasound to assist in identifying the correct spinal level for primary lumbar spinal surgery.
J Spine Surg
December 2024
Department of Neurosurgery, University Hospital of Wales, Cardiff, Wales, UK.
Lumbar spinal surgery relies on palpation of anatomical landmarks and X-ray imaging confirmation to identify the correct spinal level, therefore exposing patients and staff to radiation, and increasing intraoperative time and cost. Ultrasound (US) assistance is being used to visualise spinal anatomy by many specialities, such as neurology and anaesthetics, and can be used intraoperatively in selected spinal surgery cases. However, its potential use to check spinal levels prior to surgery remains understudied.
View Article and Find Full Text PDFThe clinicopathological significance of BRI3BP in women with invasive breast cancer.
Transl Cancer Res
December 2024
Al-Lith University College, Umm Al-Qura University, Makkah, Saudi Arabia.
Background: Invasive breast cancer (BC) is a highly life-threatening disease affecting women world-wide. While its early identification may benefit the provision of more effective therapies, several BC-associated factors may influence BC patients' therapeutic outcomes. Therefore, identifying novel prognostic and therapeutic targets for invasive BC can help with accurate prognosis and therapy-related decisions.
View Article and Find Full Text PDFOptimal extreme learning machine for diagnosing brain tumor based on modified sailfish optimizer.
Heliyon
July 2024
College of Engineering and IT, University of Dubai, Academic City, 14143, Dubai, United Arab Emirates.
This study proposes a hierarchical automated methodology for detecting brain tumors in Magnetic Resonance Imaging (MRI), focusing on preprocessing images to improve quality and eliminate artifacts or noise. A modified Extreme Learning Machine is then used to diagnose brain tumors that are integrated with the Modified Sailfish optimizer to enhance its performance. The Modified Sailfish optimizer is a metaheuristic algorithm known for efficiently navigating optimization landscapes and enhancing convergence speed.
View Article and Find Full Text PDFAccelerating the diagnosis of Chinese cblC type MMA patients by multiplex PCR sequencing method.
Pediatr Res
January 2025
Department of Neurology, Children's Hospital Affiliated to Capital Institute of Pediatrics, Beijing, China.
Background: CblC type methylmalonic aciduria (cblC disease) is the most common inborn error of vitamin B12 metabolism and due to mutations in the MMACHC gene. The earlier the diagnosis, the better the prognosis. Therefore, convenient and inexpensive detection method is needed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!