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Comparative performances of the Qvella FAST system and conventional methods for rapid identification and antibiotic susceptibility testing on monomicrobial positive blood cultures.
J Clin Microbiol
December 2024
Department of Bacteriology, Rennes University Hospital, Rennes, France.
Rapid and accurate diagnosis of sepsis is of paramount importance to reduce associated morbidity and mortality. The Qvella FAST System is a new instrument that concentrates and purifies bacteria from positive-flagged blood culture bottles (PFBCBs) to produce a "liquid" colony comparable to a subcultured colony in less than 40 min for rapid ID and calibrated antibiotic susceptibility testing (AST). In this study, we evaluated performances of the FAST System workflow and our rapid routine manual workflow (bacterial pellet obtained after lysis, cleaning, washing, and centrifugation for ID; AST by disc diffusion by direct inoculation after dilution) by comparison to the reference method based on 24-h bacterial subcultures.
View Article and Find Full Text PDFSingle torradovirus infections explain the mysterious cassava frogskin disease in the Americas.
Sci Rep
November 2024
Virology and Crop Protection Laboratory, Cassava Program, International Center for Tropical Agriculture (CIAT), Recta Cali-Palmira Km 17, Palmira, Colombia.
Cassava frogskin disease (CFSD) emerged in the Americas in the 1970s, but its causal agent has to date remained a mystery. The clonal propagation of cassava, high incidence of mixed infections, unknown alternative hosts, and root symptoms taking two or more crop cycles to develop, have made it difficult to identify the causal agent. Consequently, most studies on CFSD have produced a catalogue of pathogens occurring in affected plants.
View Article and Find Full Text PDFA high-throughput search for intracellular factors that affect RNA folding identifies proteins PepA and YagL as RNA chaperones that promote RNA remodelling.
RNA Biol
January 2024
McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.
Article Synopsis
- General RNA chaperones are proteins that help RNA molecules fold correctly and avoid misfolding, which is crucial for bacteria, especially with large and complex RNA structures.
- Researchers adapted a technique to screen for factors affecting RNA folding and identified eight proteins that influence the folding of a specific RNA ribozyme when deleted.
- Among these, YagL and PepA were found to bind RNA and assist in the folding process, with YagL being especially effective in promoting correct refolding of misfolded RNA, thanks to a specific protein domain responsible for its activity.
Cross-comparison of gut metagenomic profiling strategies.
Commun Biol
November 2024
Department of Medical Biology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
The rapid advancements in sequencing technologies and bioinformatics have enabled metagenomic research of complex microbial systems, but reliable results depend on consistent laboratory and bioinformatics approaches. Current efforts to identify best practices often focus on optimizing specific steps, making it challenging to understand the influence of each stage on microbial population analysis and compare data across studies. This study evaluated DNA extraction, library construction methodologies, sequencing platforms, and computational approaches using a dog stool sample, two synthetic microbial community mixtures, and various sequencing data sources.
View Article and Find Full Text PDFSpatially heterogeneous lipid dysregulation in tuberculous meningitis.
Neurobiol Dis
November 2024
Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address:
Tuberculous (TB) meningitis is the deadliest form of extrapulmonary TB which disproportionately affects children and immunocompromised individuals. Studies in pulmonary TB have shown that Mycobacterium tuberculosis can alter host lipid metabolism to evade the immune system. Cholesterol lowering drugs (i.
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