Objective: We sought to determine the effect of bacteria on fluorescence polarization (FPOL) testing of amniotic fluid.
Methods: Fusobacterium necrophorum and Escherichia coli were inoculated at concentrations of 10(3) and 10(6)/ml in amniotic-fluid specimens from 4 patients with no clinical or laboratory evidence of infection. The FPOL results were obtained at inoculation and again at 24 h of incubation. The results were compared using analysis of variance (ANOVA).
Results: The FPOL results from inoculated specimens were all within 2% of the uninoculated controls. The specimens incubated with bacteria showed a < 1-19% variation when compared with the time-zero uninoculated controls. However, uninoculated controls incubated for 24 h exhibited a 2-12% variation when compared with the time-zero controls, suggesting that the variation present was not secondary to the bacterial co-incubation.
Conclusions: In vitro, neither bacterial inoculation nor prolonged co-incubation influences FPOL results beyond the effect of incubation alone. FPOL appears to be an appropriate test to assess fetal lung maturity in patients in whom intraamniotic infection is a concern.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364429 | PMC |
| http://dx.doi.org/10.1155/S1064744995000408 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Symbiotic fungi alter plant resource allocation independent of water availability.
Am J Bot
January 2025
Pacific Biosciences Research Center, University of Hawai'i at Mānoa, Honolulu, HI, USA.
Premise: The ability of plants to adapt or acclimate to climate change is inherently linked to their interactions with symbiotic microbes, notably fungi. However, it is unclear whether fungal symbionts from different climates have different impacts on the outcome of plant-fungal interactions, especially under environmental stress.
Methods: We tested three provenances of fungal inoculum (originating from dry, moderate or wet environments) with one host plant genotype exposed to three soil moisture regimes (low, moderate and high).
Chickpea displays a temporal growth response to Mesorhizobium strains under well-watered and drought conditions.
Physiol Plant
January 2025
School of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA, Australia.
The relative performance of rhizobial strains could depend on their resource allocation, environmental conditions, and host genotype. Here, we used a high-throughput shoot phenotyping to investigate the effects of Mesorhizobium strain on the growth dynamics, nodulation and bacteroid traits with four chickpea (Cicer arietinum) varieties grown under different water regimes in an experiment including four nitrogen sources (two Mesorhizobium strains, and two uninoculated controls: nitrogen fertilised and unfertilised) under well-watered and drought conditions. We asked three questions.
View Article and Find Full Text PDFEffects of Nosema ceranae and Lotmaria passim infections on honey bee foraging behaviour and physiology.
Int J Parasitol
December 2024
Agriculture and Agri-Food Canada, Beaverlodge Research Farm, P.O. Box 29, Beaverlodge, Alberta, Canada T0H 0C0. Electronic address:
Nosema ceranae and Lotmaria passim are two commonly encountered digestive tract parasites of the adult honey bee (Apis mellifera L.). Although these parasites are associated with colony losses, little is known about how they affect individual bee physiology and behaviour at the colony level.
View Article and Find Full Text PDFDiversity and functional traits based indigenous rhizosphere associated phosphate solubilizing bacteria for sustainable production of rice.
Front Microbiol
December 2024
Division of Soil and Environmental Biotechnology, National Institute for Biotechnology and Genetic Engineering College (NIBGE-C), Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, Pakistan.
Introduction: Rice, particularly Basmati rice, holds significant global importance as a staple food. The indiscriminate use of phosphate-based fertilizers during rice production has led to high residual levels of these chemicals in soil, impacting soil health and fertility. This study aimed to address this challenge by investigating the potential of phosphate solubilizing bacteria (PSB) in improving soil fertility and boosting the growth of Basmati rice.
View Article and Find Full Text PDFEvaluation and identification of metabolites produced by in the interaction with plants and their effect on .
Curr Res Microb Sci
November 2024
Facultad de Agronomía y Veterinaria. Universidad Autónoma de San Luis Potosí. Soledad de Graciano Sánchez, SLP, CP, 78321. México.
Currently, the use of bio-inputs is increasing due to the need to reduce the use of agrochemicals. However, one of the limitations is to preserve the viability of the living microorganisms, so it is important to find an alternative that allows us to obtain different metabolites to produce it. We evaluated three different interactions (contact, diffusible and volatile compounds) in (At) seedlings with the strain M10 and its filtered secondary metabolites (M10F).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!