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Engineered microorganisms are currently used for the production of food products, pharmaceuticals, ethanol fuel and more. Even so, the enormous potential of this technology has yet to be fully exploited. The need for sustainable sources of transportation fuels has generated a tremendous interest in technologies that enable biofuel production. Decades of work have produced a considerable knowledge-base for the physiology and pathway engineering of microbes, making microbial engineering an ideal strategy for producing biofuel. Although ethanol currently dominates the biofuel market, some of its inherent physical properties make it a less than ideal product. To highlight additional options, we review advances in microbial engineering for the production of other potential fuel molecules, using a variety of biosynthetic pathways.
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http://dx.doi.org/10.1016/j.tibtech.2008.03.008 | DOI Listing |
Int J Biol Macromol
March 2025
National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
The rational design of enzymes represents a critical strategy for achieving efficient and sustainable biocatalysis. In this study, enzyme evolution guided by rational design was utilized to engineer two key enzymes, DAHP synthase (AroA) and prephenate dehydrogenase (TyrA), within the biosynthetic pathway of L-tyrosine. The beneficial mutants AroA and TyrA were identified, leading to a 102 % and 105 % increase in L-tyrosine yield, respectively.
View Article and Find Full Text PDFBioresour Technol
March 2025
State Key Laboratory of Petroleum Pollution Control, Xi'an Shiyou University, Xi'an, China; Shaanxi Province Key Laboratory of Environmental Pollution Control and Reservoir Protection Technology of Oilfields, Xi'an Shiyou University, Xi'an, China.
The rapid enrichment of anaerobic ammonia oxidation (anammox) bacteria (AnAOB) is challenging owing to their slow growth rate. Substrate supply strategies influence the AnAOB yield and anammox performance. In this study, a feeding strategy for low nitrogen strength with constant substrate concentrations and a hydraulic retention time decreasing over a gradient was investigated in an up-flow sludge blanket reactor.
View Article and Find Full Text PDFEnviron Res
March 2025
Institute of Energy and Environmental Protection, Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100091, China.
The effect of acidifiers on nitrogen transformation during manure wastewater storage remains unclear. In this study, we investigate the effects of two types of acidifiers on nitrogen transformation, bacterial structure, and functional genes during manure wastewater storage. The results indicate that the internal reactions in manure wastewater storage could be divided into three stages, i.
View Article and Find Full Text PDFJ Hazard Mater
March 2025
Centre for Environmental Health and Engineering (CEHE), School of Engineering, University of Surrey, Guildford GU2 7XH, United Kingdom. Electronic address:
Temperatures between 40°C and 50°C are increasingly implemented in full-scale agricultural anaerobic digestion (AD), yet the microbial diversity and antibiotic resistome dynamics within this temperature range remain poorly understood. Here, we defined this range as "hyper-mesophilic" and surveyed five full-scale sites. Significant differences were found in the bacterial community structure, potentially stemming from feedstock combination (high vs low/non-manure) and operating temperature.
View Article and Find Full Text PDFJ Hazard Mater
March 2025
Engineering Laboratory of Low-Carbon Unconventional Water Resources Utilization and Water Quality Assurance, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Efficient and eco-friendly technologies for removing antibiotic pollutants like chloramphenicol (CAP) from wastewater are becoming increasingly important. Among these, microalgal-bacterial granular sludge (MBGS) represents a promising green biotechnology capable of tackling such contaminants. However, the interactions between CAP and MBGS, as well as how CAP influences the community structure and function of MBGS, have not yet been fully understood.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!