A irreversible Hg2+ selective ratiometric fluorescence probe FR, a fluorescein fluorophore linked to a rhodamine B hydrazide by a thiourea spacer, was designed and synthesized. The developed probe FR exhibited great ratiometric fluorescence enhancement and remarkable yellow-magenta color change toward Hg2+ with excellent selectivity in aqueous acetone solution, and the ratiometric fluorescence response to Hg2+ was not interfered by other metal cations including Fe3+, Co2+, Ni2+, Cr3+, Zn2+, Pb2+, Cd2+, Ca2+, Mg2+, Ba2+ and Mn2+. The linear range and the detection limit of this supposed ratiometric fluorescence method for Hg2+ were 0.0-10.0x10(-6) and 5x10(-8) M, respectively.
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