Non-invasive fluorescent imaging of preclinical animal models in vivo is a rapidly developing field with new emerging technologies and techniques. Quantum dot (QD) fluorescent probes with longer emission wavelengths in red and near infrared (NIR) emission ranges are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased. We have designed and synthesized red CdTe and NIR CdHgTe QDs for fluorescent imaging. We demonstrated fluorescent imaging by using CdTe and CdHgTe QDs as fluorescent probes both in vitro and in vivo. Both CdTe and CdHgTe QDs provided sensitive detection over background autofluorescence in tissue biopsies and live mice, making them attractive probes for in vivo imaging extending into deep tissues or whole animals. The studies suggest a basis of using QD-antibody conjugates to detect membrane antigens.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A Sensitive and Reusable Phenothiazine-Benzophenone Based Fluorescence Probe for Detecting Hypochlorite in Environmental and Biological Systems.
J Fluoresc
January 2025
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore -14, Tamil Nadu, India.
This study addresses the critical issue of irreversible oxidation in hypochlorite (ClO⁻) sensing by a phenothiazine-based compound, which typically leads to the probe's degradation and loss of functionality. We introduce a novel fluorescence probe, (2-(5-(10 H-phenothiazin-10-yl)thiophen-2-yl)-1 H-benzo[d]imidazol-6-yl)(phenyl)methanone (PTH-BP), specifically designed to enhance ClO⁻ detection efficiency. PTH-BP exhibits strong aggregation-induced emission (AIE), emitting deep orange fluorescence at 620 nm with a large Stokes shift of 195 nm, and achieves an impressive detection limit of 1 nM in ACN/PBS buffer solutions.
View Article and Find Full Text PDFHarmonized technical standard test methods for quality evaluation of medical fluorescence endoscopic imaging systems.
Vis Comput Ind Biomed Art
January 2025
School of Engineering Medicine and School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
Fluorescence endoscopy technology utilizes a light source of a specific wavelength to excite the fluorescence signals of biological tissues. This capability is extremely valuable for the early detection and precise diagnosis of pathological changes. Identifying a suitable experimental approach and metric for objectively and quantitatively assessing the imaging quality of fluorescence endoscopy is imperative to enhance the image evaluation criteria of fluorescence imaging technology.
View Article and Find Full Text PDFEffect of Chirality and Amphiphilicity on the Antimicrobial Activity of Tripodal Lysine-Based Peptides.
ACS Appl Bio Mater
January 2025
School of Chemistry, Pharmacy and Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AD, U.K.
A series of tripodal (three-arm) lysine-based peptides were designed and synthesized and their self-assembly properties in aqueous solution and antimicrobial activity were investigated. We compare the behaviors of homochiral tripodal peptides (KKY)K and a homologue containing the bulky aromatic fluorenylmethoxycarbonyl (Fmoc) group Fmoc-(KKY)K, and heterochiral analogues containing k (d-Lys), (kkY)K and Fmoc-(kkY)K. The molecular conformation and self-assembly in aqueous solutions were probed using various spectroscopic techniques, along with small-angle X-ray scattering (SAXS) and cryogenic-transmission electron microscopy (cryo-TEM).
View Article and Find Full Text PDFTargeted NIR Fluorescent Mechanically Interlocked Molecules-Peptide Bioconjugate for Live Cancer Cells Submitochondrial Stimulated Emission Depletion Super-Resolution Microscopy.
Bioconjug Chem
January 2025
Department of Chemistry, Organic Chemistry Section, Jadavpur University, Kolkata 700032, India.
Herein, a water-soluble, ultrabright, near-infrared (NIR) fluorescent, mechanically interlocked molecules (MIMs)-peptide bioconjugate is designed with dual targeting capabilities. Cancer cell surface overexpressed αβ integrin targeting two RGDS tetrapeptide residues is tethered at the macrocycle of MIMs-peptide bioconjugate via Cu(I)-catalyzed click chemistry on the Wang resin, and mitochondria targeting lipophilic cationic TPP functionality is conjugated at the axle dye. Living carcinoma cell selective active targeting, subsequently cell penetration, mitochondrial imaging, including the ultrastructure of cristae, and real-time tracking of malignant mitochondria by MIMs-peptide bioconjugate (RGDS)-Mito-MIMs-TPP are established by stimulated emission depletion (STED) super-resolved fluorescence microscopy.
View Article and Find Full Text PDFFlexible Site-Specific Labeling-Mediated Self-Assembly Sensor Based on Quantum Dots and LUMinescent AntiBody Sensor for Duplexed Detection of Antibodies.
ACS Sens
January 2025
Materials Interfaces Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, P. R. China.
Over recent years, the LUMinescent AntiBody Sensor (LUMABS) system, utilizing bioluminescence resonance energy transfer (BRET), has emerged as a highly effective method for antibody detection. This system incorporates NanoLuc (Nluc) as the donor and fluorescent protein (FP) as the acceptor. However, the limited Stokes shift of FP poses a challenge, as it leads to significant spectral cross-talk between the excitation and emission spectra.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!