In order to determine the most effective protocol for the isolation of Arcobacter spp. from retail packs of beef, three published methods (A, B, and C) were selected. In addition, a modified version of method B was studied (method D). The ability of the four methods to isolate Arcobacter from standardized beef samples (n = 80) was compared with presumptive Arcobacter isolates being identified to genus and species level, using multiplex PCR methods. The presence of Arcobacter in enrichment broths was also investigated using PCR techniques. Overall, the modified enrichment and selection media of Johnson and Murano (method D) gave the highest recovery of Arcobacter. Recovery using these media was enhanced by incubating the enrichment and selection media in a microaerobic cabinet rather than air, and the inclusion of streaking the enrichment broth onto selective agar after 24 h in addition to 48 h. Method D yielded significantly more Arcobacter-positive samples of beef (P < 0.01) than did the three other methods investigated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028x-71.4.850 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effectiveness of gaseous ozone on and bacterial loads on retailed meat sold at Iraqi Wasit markets.
Open Vet J
November 2024
Department of Nursing, Technical Institute of Suwaria, Middle Technical University, Baghdad, Iraq.
Background: Ozone (O) is a promising alternative antibacterial agent that has recently been used in meat processing. The understanding of the appropriate functional settings of O for addressing food safety problems is still insufficient.
Aim: The aim of this study was, therefore, to investigate the effects of exposure to O on the bacteriological quality of retail meat inoculated with at refrigeration temperatures.
Deciphering the gut microbiota's role in diverticular disease: insights from a Mendelian randomization study.
Front Cell Infect Microbiol
December 2024
Department of Gastrointestinal Surgery, Longyan First Hospital, Longyan, China.
Article Synopsis
- Previous research suggested a link between gut microbiota and diverticular disease, but the exact nature wasn’t well-defined, prompting this study using a Mendelian randomization approach.
- The study involved genetic data from a large sample of European participants to explore how specific gut microbes may influence the risk of developing intestinal diverticular disease.
- Results identified 11 microbial taxa with potential causal relationships to the disease—some increasing risk while others decreased it—offering insights for future prevention and treatment strategies.
Dynamic Changes in Microbiome and Metabolome during Sun-Drying of Oysters (), a Traditional Procedure in South China.
J Microbiol Biotechnol
December 2024
Department of Food Science, Foshan Polytechnic, Foshan 528137, P.R. China.
Sun-drying constitutes a traditional method employed in the preparation of dried oysters within the coastal regions of South China. However, its ramifications on nutritional attributes and the genesis of flavor-contributory compounds in the resultant dried oysters remain significantly unexplored. This research endeavors to scrutinize the repercussions of the production process on the microbiota and metabolites within dried oysters.
View Article and Find Full Text PDFArcobacter species isolated from human stool samples, animal products, ready-to-eat salad mixes, and ambient water: prevalence, antimicrobial susceptibility, and virulence gene profiles.
BMC Infect Dis
November 2024
Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Introduction: Arcobacter species are emerging foodborne pathogens increasingly associated with human illness worldwide. They are commonly found in the gastrointestinal tracts of animals and are frequently isolated from various food sources, including raw meat, poultry, and seafood. The aim of this study is to investigate the antimicrobial resistance patterns of Arcobacter spp.
View Article and Find Full Text PDFDepuration of and in Comparison with in Mussels () and Oysters ().
Pathogens
November 2024
Aquaculture, A Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Centre de la Ràpita, 43540 la Ràpita, Catalonia, Spain.
-related species are considered emerging food-borne and waterborne pathogens, with shellfish being a suggested reservoir. In a published study that investigated 204 shellfish samples and 476 isolates, the species (now known as ) and (now known as ) have been isolated as the most dominant species. However, the efficiency of depuration for eliminating and in comparison with from mussels and oysters is unknown and is therefore the objective of this investigation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!