AI Article Synopsis
- The study aimed to investigate how extracts of huangqi and dangshen (referred to as SQ) affect the formation of new blood vessels (angiogenesis) triggered by b-FGF.
- Mice underwent an experiment where a substance containing b-FGF was injected, and the results were assessed by measuring hemoglobin levels and the area of new blood vessels formed in the implanted material after administering different doses of SQ or a control solution.
- Results showed that lower doses of SQ promoted angiogenesis, while higher doses inhibited it, indicating that the concentration of SQ significantly influences its effect on blood vessel formation.
Article Abstract
Aim: To study the inhibitory effect of huangqi and dangshen extraction (SQ) on angiogenesis induced by b-FGF.
Methods: Matrigel implant assay was used. Matrigel(500 microL) containing b-FGF and heparin was injected subcutaneously into the abdomens of mice and harvested 5 d later. The amount of hemoglobin and micro-vascular area present in the implant were measured and compared. The mice were given different dosage of SQ (experimental group) or the same volume of glucose (vehicle group) once a day by intraperitoneal injection. Inhibitory experiment started 3 d before Matrigel implant and continued until the end of study.
Results: SQ in lower dosage (< or = 50% V/V) increased hemoglobin content and micro-vascular area in Matrigel implant while SQ in higher dosage (> or = 60%, V/V) reduced hemoglobin content and micro-vascular area in Matrigel implant. The effect of enhance ment and inhibition was in a limited concentration-effect manner.
Conclusion: SQ in different dosage has different effects on angiogenesis. We should use different dosage in different purpose.
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