AI Article Synopsis
- SARS is a serious infectious disease caused by the SARS-CoV coronavirus, with a notable mortality rate of 9.56%, but its exact disease mechanism remains unclear.
- Researchers used various laboratory techniques, including ELISA and tissue analysis, to investigate the presence of specific antibodies in SARS patients’ blood during the early phase of the illness.
- They discovered antibodies targeting the human LINE1 endonuclease protein in 40.9% of SARS patients, suggesting these autoantibodies could play a role in the disease’s development.
Article Abstract
Background: Severe acute respiratory syndrome (SARS) is a disease with a mortality of 9.56%. Although SARS is etiologically linked to a new coronavirus (SARS-CoV) and functional cell receptor has been identified, the pathogenesis of the virus infection is largely unclear.
Methods: The clinical specimens were processed and analyzed using an indirect enzyme-linked immunosorbent assay (ELISA) in-house. Further investigations of target antigen included reviews of phage display technique, rapid amplification of cDNA ends (RACE) technique, protein expression and purification, Western blotting validation, serological and immunohistochemical staining in postmortem tissue.
Results: A type of medium or low titer anti-lung tissue antibodies were found in the sera of SARS patients at the early stage of the disease. Human long interspersed nuclear element 1 (LINE1) gene endonuclease (EN) domain protein was one of the target autoantigens and it was aberrantly expressed in the lung tissue of SARS patients. Anti-EN antibody was positive in the sera of 40.9% of SARS patients.
Conclusions: Human LINE1 endonuclease domain was identified as a putative target of SARS-associated autoantibodies, which were presented in the serum of SARS patients and may be involved in the pathogenesis of SARS.
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