AI Article Synopsis
- Monitoring gene expression is essential for studying bone development (osteogenesis) and improving gene therapy for bones.
- Traditional molecular tools provided limited analysis of protein expression either in lab settings (in vitro) or in living organisms (in vivo).
- Bioluminescent imaging is highlighted as the only effective method for quantifying gene expression in both environments, particularly using transgenic mice with a luciferase gene linked to a bone-specific promoter.
Article Abstract
Monitoring gene expression in vitro and in vivo, is crucial when analyzing osteogenesis and developing effective bone gene therapy protocols. Until recently, molecular analytical tools were only able to detect protein expression either in vitro or in vivo. These systems include histology and immunohistochemistry, fluorescent imaging, PET (micro-PET), CT (micro-CT), and bioluminescent imaging. The last is the only system to date that can enable efficient quantitative monitoring of gene expression both in vitro and in vivo. Effective bioluminescent imaging in bone can be achieved by using transgenic mice harboring the luciferase reporter gene, downstream of an osteogenesis specific promoter. The aim of this chapter is to comprehensively describe the various protocols needed for the detection of bioluminescence in bone development and repair.
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| http://dx.doi.org/10.1007/978-1-59745-104-8_18 | DOI Listing |
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