Endostatin (ES), as an angiogenesis inhibitor, has been approved by the State Food and Drug Administration (SFDA) in China for the treatment of patients with non-small-cell lung cancer. However, as a protein drug, there are a lot of obstacles on its clinical application, such as need of high dose to maintain its efficacy, expensive and poor stability, etc and limits its clinical use. In order to overcome these shortcomings, we chemically modified ES by polyethylene glycol and low molecular weight heparin (LMWH), respectively. The changes of the secondary structure of the modified products were studied by Fourier transform infrared spectroscopy and Circular dichroism spectra to obtain better ES derivatives. Our study demonstrated that the modified products have a better heat tolerance than ES towards. The result of secondary structure analysis suggests the percentage of beta-turn in whole protein is an important factor on the activity and heat stability and ES modified by LMWH can maintain higher activity and its secondary structure.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1093/jb/mvn060 | DOI Listing |
Nat Commun
January 2025
Center for High Pressure Science, State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao, 066004, China.
Hydrous aluminosilicates are important deep water-carriers in sediments subducting into the deep mantle. To date, it remains enigmatic how hydrous aluminosilicates withstand extremely high temperatures in the mantle transition zone. Here we systematically investigate the crystal structures and chemical compositions of typical hydrous aluminosilicates using single-crystal X-ray diffraction, electron probe microanalyzer, and nanoscale secondary ion mass spectrometry.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
Food Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518045, PR China.
Background: Ochratoxin A (OTA) is toxic secondary metabolites produced by fungi and can pose a serious threat to food safety and human health. Due to the high stability and toxicity, OTA contamination in agricultural products is of great concern. Therefore, the development of a highly sensitive and reliable OTA detection method is crucial to ensure food safety.
View Article and Find Full Text PDFCancer Lett
January 2025
. Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China. Electronic address:
Tertiary lymphoid structures (TLSs) are ectopic immune cell clusters formed in nonlymphoid tissues affected by persistent inflammation, such as in cancer and prolonged infections. They have features of the structure and function of secondary lymphoid organs, featuring central CD20+ B cells, surrounded by CD3+ T cells, CD21+ follicular dendritic cells, and CD68+ macrophages, with a complex vascular system. TLS formation is governed by lymphotoxin-α1β2, TNF, and chemokines like CCL19, CCL21, and CXCL13, differing from secondary lymphoid organ development in developing later in life at sites of chronic inflammation.
View Article and Find Full Text PDFNat Prod Res
January 2025
Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, P. R. China.
A new hydrindane derivative, asperhydrindane A (), along with two known sterol analogues [isocyathisterol () and ganodermasides D ()] were isolated from the mangrove-derived fungus GXIMD 03158 attaching to the mangrove L. The structure of was elucidated based on extensive spectral analysis, HRESIMS, and calculated ECD methods. All compounds were evaluated for antibacterial activity.
View Article and Find Full Text PDFPharmaceutics
January 2025
School of Medicine and Population Health, The University of Sheffield, Barber House, Sheffield S10 2HQ, UK.
: In the quest for sustainable and biocompatible materials, silk fibroin (SF), derived from natural silk, has emerged as a promising candidate for nanoparticle production. This study aimed to fabricate silk fibroin particles (SFPs) using a novel swirl mixer previously presented by our group, evaluating their characteristics and suitability for drug delivery applications, including magnetic nanoparticles and dual-drug encapsulation with curcumin (CUR) and 5-fluorouracil (5-FU). : SFPs were fabricated via microfluidics-assisted desolvation using a swirl mixer, ensuring precise mixing kinetics.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!