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Impact of algicidal fungus Aspergillus welwitschiae GF6 on harmful bloom-forming cyanobacterium Microcystis aeruginosa: Growth and physiological responses.
Chemosphere
January 2025
St. Petersburg Federal Research Center of the Russian Academy of Sciences (SPC RAS), Scientific Research Centre for Ecological Safety of the Russian Academy of Sciences, 18, Korpusnaya st., St. Petersburg, 197110, Russia.
Harmful cyanobacterial blooms (HCB) have become a common issue in freshwater worldwide. Biological methods for controlling HCB are relatively cost effective and environmentally friendly. The strain of ascomycete GF6 was isolated from a water sample collected from the estuarine zone of the eastern part of the Gulf of Finland.
View Article and Find Full Text PDFUltrasensitive "Hunter of Target": Rabbit Monoclonal Antibody-Based Competitive Lateral Flow Immunoassay for Saxitoxin.
Anal Chem
January 2025
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
The rapid, sensitive, and accurate detection of paralytic shellfish toxins (PSTs), such as saxitoxin (STX), is critical for protecting human health due to the frequent occurrence of toxic red tides. In this work, to address the low affinity of traditional mouse monoclonal antibodies (m-mAbs), rabbit monoclonal antibodies (r-mAbs) against STX were produced by a single B-cell sorting culture and a cross-selection strategy. The r-mAbs showed 100-fold improvement in sensitivity (IC = 0.
View Article and Find Full Text PDFRapid microcystin-LR detection using antibody-based electrochemical biosensors with a simplified calibration curve approach.
Sci Rep
December 2024
Department of Civil, Environmental and Construction Engineering, University of Central Florida, Orlando, FL, USA.
Harmful algal blooms (HABs) can release cyanotoxins such as microcystins (MCs), especially, microcystin-leucine-arginine (MC-LR) which is one of the commonest and most toxic, into our water bodies and can lead to several acute or chronic diseases such as liver diseases and respiratory irritation in humans. There is an increasing need for rapid and simple detection of MC-LR in water bodies for early warning of HABs. In this study, we developed an innovative on-site screening electrochemical impedance spectroscopy (EIS) biosensor with a simplified calibration curve that can rapidly detect blooms for early action in similar water bodies.
View Article and Find Full Text PDFTunable aptamer-MXene sensing interface for label-free and real-time detection of toxic pollutants in water samples.
Biosens Bioelectron
March 2025
Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
Stable and low-cost field-effect transistor (FET)-based biosensors are vital for the on-site detection of toxic pollutants in environmental monitoring applications. In this study, a tunable aptamer-MXene sensing interface was constructed to develop renewable FET biosensors. This was achieved through the reversible disulfide bond (-S-S-) reaction between the SH-TiCT film and thiolated aptamer.
View Article and Find Full Text PDFIdentification and occurrence of microcystins in freshwaters and fish from a eutrophic dam through LC-HRMS.
Sci Total Environ
January 2025
Analytical Chemistry Research Group (FQM-323), Department of Physical and Analytical Chemistry, University of Jaen, 23071 Jaén, Spain; University Research Institute for Olives Grove and Olive Oil, University of Jaen, Jaén, Spain.
Microcystins (MCs) are cyclic heptapeptides originating from various cyanobacteria in eutrophic aquatic environments. Their potential consequences on ecosystems and public health underscores the need to explore MCs' occurrence. In this study, liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) analysis and a suspect screening workflow supported by open-source tools were employed for the determination of MCs in freshwater and biota samples from a eutrophic dam in Uruguay.
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