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Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays. | LitMetric
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Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays.

Shinji Fukuda Yukie Sasaki Masato Seno

Appl Environ Microbiol

Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan.

Published: June 2008

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446545PMC
http://dx.doi.org/10.1128/AEM.00127-08DOI Listing

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